Phylogenetic analysis and heterologous expression of surface layer protein slpc of bacillus sphaericus c3-41
文献类型：期刊论文
| 作者 | Hu, Xiaomin1; Li, Jia1; Hansen, Bjarne Munk2; Yuan, Zhiming1 |
| 刊名 | Bioscience biotechnology and biochemistry
 |
| 出版日期 | 2008-05-01 |
| 卷号 | 72期号:5页码:1257-1263 |
| 关键词 | Surface layer Bacillus sphaericus Pathogenic Crystal lattice Toxicity |
| ISSN号 | 0916-8451 |
| DOI | 10.1271/bbb.70747 |
| 通讯作者 | Yuan, zhiming(yzm@wh.iov.cn) |
| 英文摘要 | The surface layer protein encoding genes from five mosquito-pathogenic bacillus sphaericus isolates were amplified and sequenced. negative staining of the slayer protein extracted from the cell wall of wild-type b. sphaericus c3-41 was prepared. it showed a flat-sheet crystal lattice structure. two genes encoding the entire and n-terminally truncated s-layer protein (slpc and delta slpc respectively), were ligated into plasmid pet28a and expressed in escherichia coli. sds-page revealed that about 130kd and 110kd proteins could be expressed in the cytoplasm of recombinant e. coli bl21(pet28a/slpc) and e. coli bl21(pet28a/delta slpc) respectively. furthermore, an intracellular sheet-like or fingerprint-shape structure was investigated in two recombinant strains, which expressed slpc and delta slpc protein respectively, by ultrathin microscopy study, but bioassay results suggested that the s-layer protein of wild b. sphaericus c3-41 and recombinant e. coli bl21 (pet28a/slpc) have no direct toxicity against mosquito larvae. these results should provide information for further understanding of the function of s-layer protein of pathogenic b. sphaericus. |
| WOS关键词 | S-LAYER ; STEAROTHERMOPHILUS ATCC-12980 ; CULEX-QUINQUEFASCIATUS ; STRAINS ; TOXICITY ; URANIUM ; CELLS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry, Applied ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000256270600015 |
| 出版者 | TAYLOR & FRANCIS LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375441 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yuan, Zhiming |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Natl Environm Res Inst, DK-4000 Roskilde, Denmark |
| 推荐引用方式 GB/T 7714 | Hu, Xiaomin,Li, Jia,Hansen, Bjarne Munk,et al. Phylogenetic analysis and heterologous expression of surface layer protein slpc of bacillus sphaericus c3-41[J]. Bioscience biotechnology and biochemistry,2008,72(5):1257-1263. |
| APA | Hu, Xiaomin,Li, Jia,Hansen, Bjarne Munk,&Yuan, Zhiming.(2008).Phylogenetic analysis and heterologous expression of surface layer protein slpc of bacillus sphaericus c3-41.Bioscience biotechnology and biochemistry,72(5),1257-1263. |
| MLA | Hu, Xiaomin,et al."Phylogenetic analysis and heterologous expression of surface layer protein slpc of bacillus sphaericus c3-41".Bioscience biotechnology and biochemistry 72.5(2008):1257-1263. |
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来源：武汉病毒研究所
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