Split mcherry as a new red bimolecular fluorescence complementation system for visualizing protein-protein interactions in living cells
文献类型：期刊论文
| 作者 | Fan, Jin-Yu1,2; Cui, Zong-Qiang1; Wei, Hong-Ping1; Zhang, Zhi-Ping1; Zhou, Ya-Feng1; Wang, Yun-Peng1; Zhang, Xian-En1 |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2008-02-29 |
| 卷号 | 367期号:1页码:47-53 |
| 关键词 | Bimolecular fluorescence complementation Bifc Mcherry Protein-protein interactions Red fluorescent protein |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2007.12.101 |
| 通讯作者 | Zhang, xian-en(x.zhang@wh.iov.cn) |
| 英文摘要 | Bimolecular fluorescence complementation (bifc) is a recently developed technique for detection of protein-protein interactions in living cells. in this study, a new red bifc system was developed by splitting mcherry, a mutant monomeric red fluorescent protein, into two fragments between amino acids 159-160 and was verified using a pair of interacting proteins, sv40 large t antigen (ltag), and human p53 protein. by combined use of the mcherry-based red bifc system with a venus-based yellow bifc system, the interaction between ltag and p53 as well as the interaction between sp100 and promyelocytic leukemia protein (pml), were detected simultaneously in vero cells. the brilliant redness, short maturation time, and the long excitation and emission wavelengths (587/610 nm) of mcherry make the new bifc system an excellent candidate for analyzing protein-protein interactions in living cells and for studying multiple protein-protein interactions when coupled with other bifc systems. (c) 2007 elsevier inc. all rights reserved. |
| WOS关键词 | IN-VIVO ; FRAGMENT COMPLEMENTATION ; MOLECULAR-INTERACTIONS ; MAMMALIAN-CELLS ; DYNAMICS ; BIFC ; FRET ; P53 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000252619700008 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375442 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Xian-En |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Hubei Bioinformat & Mol Imaging Key Lab, Minist Educ,Key Lab Biomed Photon, Wuhan 430074, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Jin-Yu,Cui, Zong-Qiang,Wei, Hong-Ping,et al. Split mcherry as a new red bimolecular fluorescence complementation system for visualizing protein-protein interactions in living cells[J]. Biochemical and biophysical research communications,2008,367(1):47-53. |
| APA | Fan, Jin-Yu.,Cui, Zong-Qiang.,Wei, Hong-Ping.,Zhang, Zhi-Ping.,Zhou, Ya-Feng.,...&Zhang, Xian-En.(2008).Split mcherry as a new red bimolecular fluorescence complementation system for visualizing protein-protein interactions in living cells.Biochemical and biophysical research communications,367(1),47-53. |
| MLA | Fan, Jin-Yu,et al."Split mcherry as a new red bimolecular fluorescence complementation system for visualizing protein-protein interactions in living cells".Biochemical and biophysical research communications 367.1(2008):47-53. |
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来源：武汉病毒研究所
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