Green tea extract and its major component epigallocatechin gallate inhibits hepatitis b virus in vitro
文献类型：期刊论文
| 作者 | Xu, Jun1,2; Wang, Jue1; Deng, Fei1; Hu, Zhihong1; Wang, Hualin1 |
| 刊名 | Antiviral research
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 78期号:3页码:242-249 |
| 关键词 | Hepatitis b virus Green tea extract Epigallocatechin gallate Hbeag Hbsag hbv dna Repliction intermediates Cccdna |
| ISSN号 | 0166-3542 |
| DOI | 10.1016/j.antiviral.2007.11.011 |
| 通讯作者 | Wang, hualin(h.wang@wh.iov.cn) |
| 英文摘要 | Hepatitis b virus (hbv) infection is endemic in asia and causes major public health problems worldwide. present treatment strategies for hbv infections are not satisfactory and the clinical limitation of current antiviral drugs for hbv, such as lamivudine, is causing rapid emergence of drug-resistant viral strains during the prolonged therapeutic treatment. in this research, the efficacy of a natural green tea extract (gte) against hbv in a stably expressed hbv cell line hepg2-n10 is examined. the expression of viral antigens, hbsag and hbeag, were determined by using enzyme linked immuno-absorbent assay (elisa). quantitative real-time-pcr (q-pcr) was used for the determination of extracellular hbv dna and intracellular replicative intermediates and nuclear covalent closed circular dna (cccdna). hbv mrnas were also analyzed by reverse transcription pcr (rt-pcr). results showed that the 50% effective concentration (ec50) of gte on hbsag, hbeag, extracellular hbv dna and intracellular hbv dna were 5.02, 5.681, 19.81, and 10.76 mu g/ml, respectively. while the concentration of gte with the inhibition percentage of 50% on proliferating cells (cc50) was 171.8 mu g/ml. similar analysis of the principal component of gte, epigallocatechin gallate (egcg), revealed it has relative weaker efficacy compared to gte. (c) 2008 elsevier b.v. all rights reserved. |
| WOS关键词 | (-)-EPIGALLOCATECHIN GALLATE ; INFLUENZA-VIRUS ; X-PROTEIN ; DNA ; REPLICATION ; CATECHINS ; CELLS ; ANGIOGENESIS ; POLYPHENOL ; RESISTANCE |
| WOS研究方向 | Pharmacology & Pharmacy ; Virology |
| WOS类目 | Pharmacology & Pharmacy ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000255383300008 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375453 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Hualin |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Jun,Wang, Jue,Deng, Fei,et al. Green tea extract and its major component epigallocatechin gallate inhibits hepatitis b virus in vitro[J]. Antiviral research,2008,78(3):242-249. |
| APA | Xu, Jun,Wang, Jue,Deng, Fei,Hu, Zhihong,&Wang, Hualin.(2008).Green tea extract and its major component epigallocatechin gallate inhibits hepatitis b virus in vitro.Antiviral research,78(3),242-249. |
| MLA | Xu, Jun,et al."Green tea extract and its major component epigallocatechin gallate inhibits hepatitis b virus in vitro".Antiviral research 78.3(2008):242-249. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。