Virus-like particles of sars-like coronavirus formed by membrane proteins from different origins demonstrate stimulating activity in human dendritic cells
文献类型：期刊论文
| 作者 | Bai, Bingke1,3,4; Hu, Qinxue1; Hu, Hui1; Zhou, Peng1,2; Shi, Zhengli1; Meng, Jin1,2; Lu, Baojing1,2; Huang, Yi1; Mao, Panyong3; Wang, Hanzhong1 |
| 刊名 | Plos one
 |
| 出版日期 | 2008-07-16 |
| 卷号 | 3期号:7页码:12 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0002685 |
| 通讯作者 | Bai, bingke() |
| 英文摘要 | The pathogenesis of sars coronavirus (cov) remains poorly understood. in the current study, two recombinant baculovirus were generated to express the spike (s) protein of sars-like coronavirus (sl-cov) isolated from bats (vacbs) and the envelope (e) and membrane (m) proteins of sars-cov, respectively. co-infection of insect cells with these two recombinant baculoviruses led to self-assembly of virus- like particles (bvlps) as demonstrated by electron microscopy. incorporation of s protein of vacbs (bs) into vlps was confirmed by western blot and immunogold labeling. such bvlps up-regulated the level of cd40, cd80, cd86, cd83, and enhanced the secretion of il-6, il-10 and tnf-alpha in immature dendritic cells (dcs). immune responses were compared in immature dcs inoculated with bvlps or with vlps formed by s, e and m proteins of human sars-cov. bvlps showed a stronger ability to stimulate dcs in terms of cytokine induction as evidenced by 2 to 6 fold higher production of il-6 and tnf-alpha. further study indicated that ifn-gamma+ and il-4+ populations in cd4+ t cells increased upon co-cultivation with dcs pre-exposed with bvlps or sars-cov vlps. the observed difference in dc- stimulating activity between bvlps and sars cov vlps was very likely due to the s protein. in agreement, sl-cov s dna vaccine evoked a more vigorous antibody response and a stronger t cell response than sars-cov s dna in mice. our data have demonstrated for the first time that sl-cov vlps formed by membrane proteins of different origins, one from sl-cov isolated from bats (bs) and the other two from human sars-cov (e and m), activated immature dcs and enhanced the expression of costimulatory molecules and the secretion of cytokines. finding in this study may provide important information for vaccine development as well as for understanding the pathogenesis of sars-like cov. |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000264057200029 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375474 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Bai, Bingke |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Inst Infect Dis, Dept Virol, Beijing, Peoples R China 4.Chinese Peoples Liberat Army, Postgrad Med Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Bingke,Hu, Qinxue,Hu, Hui,et al. Virus-like particles of sars-like coronavirus formed by membrane proteins from different origins demonstrate stimulating activity in human dendritic cells[J]. Plos one,2008,3(7):12. |
| APA | Bai, Bingke.,Hu, Qinxue.,Hu, Hui.,Zhou, Peng.,Shi, Zhengli.,...&Wang, Hanzhong.(2008).Virus-like particles of sars-like coronavirus formed by membrane proteins from different origins demonstrate stimulating activity in human dendritic cells.Plos one,3(7),12. |
| MLA | Bai, Bingke,et al."Virus-like particles of sars-like coronavirus formed by membrane proteins from different origins demonstrate stimulating activity in human dendritic cells".Plos one 3.7(2008):12. |
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来源：武汉病毒研究所
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