Distribution of norovirus in oyster tissues
文献类型：期刊论文
| 作者 | Wang, Dapeng1,2,3; Wu, Qingping1; Kou, Xiaoxia1; Yao, Lin1,2,3; Zhang, Jumei1 |
| 刊名 | Journal of applied microbiology
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 105期号:6页码:1966-1972 |
| 关键词 | Distribution Norovirus Oyster tissues |
| ISSN号 | 1364-5072 |
| DOI | 10.1111/j.1365-2672.2008.03970.x |
| 通讯作者 | Wu, qingping(wuqp@gdas.ac.cn) |
| 英文摘要 | To study the distribution of norovirus (nv) in oyster tissues. five monoclonal antibodies (mabs) against vp1 were selected from balb/c mice. immunohistochemical analysis was performed to detect native nv in different tissues of artificially contaminated oysters using the mabs. the data showed that the gills and the digestive glands are efficient tissues for accumulation of the nv. in addition, the nv was found on the cilia of the mantle after filtering. in our study, only nv rna in the gills could be detected by reverse transcriptase-polymerase chain reaction. nv was bioaccumulated in the gills, stomach, digestive diverticula and cilia of the mantle. furthermore, the results suggested that the viral load of the gills and the digestive glands is heavier than that of the other tissues. this, to our knowledge, is the first paper to report the distribution of nv in oyster tissues by immunoassay after artificial contamination. further understanding of the nv distribution in oyster may help us to sample appropriate tissues for detection of the virus. |
| WOS关键词 | HEPATITIS-A VIRUS ; NORWALK VIRUS ; RT-PCR ; SHELLFISH TISSUES ; RAPID DETECTION ; GASTROENTERITIS ; OUTBREAKS ; SEQUENCE ; BINDING ; SAMPLES |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000261125300025 |
| 出版者 | WILEY-BLACKWELL PUBLISHING, INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375480 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wu, Qingping |
| 作者单位 | 1.Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, Guangzhou 510070, Guangdong, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Dapeng,Wu, Qingping,Kou, Xiaoxia,et al. Distribution of norovirus in oyster tissues[J]. Journal of applied microbiology,2008,105(6):1966-1972. |
| APA | Wang, Dapeng,Wu, Qingping,Kou, Xiaoxia,Yao, Lin,&Zhang, Jumei.(2008).Distribution of norovirus in oyster tissues.Journal of applied microbiology,105(6),1966-1972. |
| MLA | Wang, Dapeng,et al."Distribution of norovirus in oyster tissues".Journal of applied microbiology 105.6(2008):1966-1972. |
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来源：武汉病毒研究所
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