Evaluation of different n-glycopeptide enrichment methods for n-glycosylation sites mapping in mouse brain
文献类型：期刊论文
| 作者 | Zhang, Chengqian1,2,3; Ye, Zilu1,2,3; Xue, Peng1,2; Shu, Qingbo1,2,3; Zhou, Yue1,2,3; Ji, Yanlong1,2,3; Fu, Ying1,2,3; Wang, Jifeng1,2; Yang, Fuquan1,2,3 |
| 刊名 | Journal of proteome research
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 15期号:9页码:2960-2968 |
| ISSN号 | 1535-3893 |
| 关键词 | N-glycosylation sites Zic-hilic Lectins Tio2 Hydrazide Mouse brain |
| DOI | 10.1021/acs.jproteome.6b00098 |
| 通讯作者 | Yang, fuquan(fqyang@ibp.ac.cn) |
| 英文摘要 | N-glycosylation of proteins plays a critical role in many biological pathways. because highly heterogeneous n-glycopeptides are present in biological sources, the enrichment procedure is a crucial step for mass spectrometry analysis. five enrichment methods, including ip-zic-hilic, hydrazide chemistry, lectin affinity, zic-hilic-fa, and tio2 affinity were evaluated and compared in the study of mapping n-glycosylation sites in mouse brain. on the basis of our results, the identified n-glycosylation sites were 1891, 1241, 891, 869, and 710 and the fdr values were 3.29, 5.62, 9.54, 9.54, and 20.02%, respectively. therefore, ip-zic-hilic enrichment method displayed the highest sensitivity and specificity. in this work, we identified a total of 3446 unique glycosylation sites conforming to the n-glycosylation consensus motif (n-x-t/s/c; x not equal p) with o-18 labeling in 1597 n-glycoproteins. n-glycosylation site information was used to confirm or correct the transmembrane topology of the 57 novel transmembrane n-glycoproteins. |
| WOS关键词 | SOLID-PHASE EXTRACTION ; HYDROPHILIC INTERACTION CHROMATOGRAPHY ; TITANIUM-DIOXIDE CHROMATOGRAPHY ; TRANSMEMBRANE PROTEIN TOPOLOGY ; MASS-SPECTROMETRY ; HYDRAZIDE CHEMISTRY ; LINKED GLYCOPEPTIDES ; IDENTIFICATION ; MEMBRANE ; DATABASE |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemical Research Methods |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000382713300007 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375481 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yang, Fuquan |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Lab Prot & Peptide Pharmaceut, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Lab Prote, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Chengqian,Ye, Zilu,Xue, Peng,et al. Evaluation of different n-glycopeptide enrichment methods for n-glycosylation sites mapping in mouse brain[J]. Journal of proteome research,2016,15(9):2960-2968. |
| APA | Zhang, Chengqian.,Ye, Zilu.,Xue, Peng.,Shu, Qingbo.,Zhou, Yue.,...&Yang, Fuquan.(2016).Evaluation of different n-glycopeptide enrichment methods for n-glycosylation sites mapping in mouse brain.Journal of proteome research,15(9),2960-2968. |
| MLA | Zhang, Chengqian,et al."Evaluation of different n-glycopeptide enrichment methods for n-glycosylation sites mapping in mouse brain".Journal of proteome research 15.9(2016):2960-2968. |
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来源：中国科学院大学
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