Hepatitis c virus (hcv) ns2 protein up-regulates hcv ires-dependent translation and down-regulates ns5b rdrp activity
文献类型：期刊论文
| 作者 | She, Yinglong1; Liao, Qingjiao2; Chen, Xulin2; Ye, Linbai1; Wu, Zhenghui1 |
| 刊名 | Archives of virology
 |
| 出版日期 | 2008-11-01 |
| 卷号 | 153期号:11页码:1991-1997 |
| ISSN号 | 0304-8608 |
| DOI | 10.1007/s00705-008-0198-3 |
| 通讯作者 | Ye, linbai(linbaiye@whu.edu.cn) |
| 英文摘要 | Chronic hepatitis c virus (hcv) infection often leads to liver cancer. the hcv ns2 protein is a hydrophobic transmembrane protein that associates with several cellular proteins in mammalian cells. in this report, we investigated the function of ns2 protein on hcv replication and translation by using a transient cell-based expression system. cells co-transfected with pcdna3.1 (-)-ns2 and the dual-luciferase reporter construct containing the hcv ires were used to detect the effect of ns2 protein on hcv translation. cells co-transfected with pcdna3.1(-)-ns2, pcdna-ns5b and a reporter plasmid were used to detect the effect of ns2 protein on hcv replication. the results showed that hcv ns2 protein up-regulated hcv ires-dependent translation in a specific and dose-dependent manner in huh7 cells but not in hela and hepg2 cells, and ns2 protein inhibited ns5b rdrp activity in a dose-independent manner in all three cell lines. these findings may suggest a novel mechanism by which hcv modulates its ns5b replication and ires-dependent translation and facilitates virus persistence. |
| WOS关键词 | CAP-INDEPENDENT TRANSLATION ; 5' NONTRANSLATED REGION ; RIBOSOMAL ENTRY SITE ; CORE PROTEIN ; NONSTRUCTURAL PROTEINS ; 3'-UNTRANSLATED REGION ; GENE-EXPRESSION ; RNA REPLICATION ; CELL-LINES ; IN-VIVO |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000260835200001 |
| 出版者 | SPRINGER WIEN |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375489 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Ye, Linbai |
| 作者单位 | 1.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | She, Yinglong,Liao, Qingjiao,Chen, Xulin,et al. Hepatitis c virus (hcv) ns2 protein up-regulates hcv ires-dependent translation and down-regulates ns5b rdrp activity[J]. Archives of virology,2008,153(11):1991-1997. |
| APA | She, Yinglong,Liao, Qingjiao,Chen, Xulin,Ye, Linbai,&Wu, Zhenghui.(2008).Hepatitis c virus (hcv) ns2 protein up-regulates hcv ires-dependent translation and down-regulates ns5b rdrp activity.Archives of virology,153(11),1991-1997. |
| MLA | She, Yinglong,et al."Hepatitis c virus (hcv) ns2 protein up-regulates hcv ires-dependent translation and down-regulates ns5b rdrp activity".Archives of virology 153.11(2008):1991-1997. |
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来源：武汉病毒研究所
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