Specific activity of a bacillus thuringiensis strain against locusta migratoria manilensis
文献类型：期刊论文
| 作者 | Song, Lingli; Gao, Meiying; Dai, Shunying; Wu, Yan; Yi, Dan; Li, Rongsen |
| 刊名 | Journal of invertebrate pathology
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 98期号:2页码:169-176 |
| 关键词 | Bacillus thuringiensis Locusta migratoria manilensis Locust Delta-endotoxin |
| ISSN号 | 0022-2011 |
| DOI | 10.1016/j.jip.2008.02.006 |
| 通讯作者 | Gao, meiying(rnygao@wh.iov.cn) |
| 英文摘要 | Bacillus thuringiensis (bt) has played an important role in biocontrol of pests. however, insecticidal activity of b. thuringiensis against locusts has been rarely reported. bt strain bth-13 exhibiting specific activity to locusts was isolated from a soil sample in china and characterized. its bipyramidal parasporal crystal is mainly composed of a protein of 129 kda, and produces a mature toxin of 64 kda after activation. the pattern of total dna from bth-13 showed a large and three small plasmid bands. known delta-endotoxin genes, cry1aa, cry1ab, cry1ac, cry1c, cry3, cry4 and cry7aa were not found from strain bth-13 by pcr amplification. the sequence analysis of a dna fragment produced by pcr amplification with degenerate cry-selective primers revealed that the fragment encoded a delta-endotoxin segment, which exhibited some similarity to several cry proteins (41% of the highest similarity to cry7ba1). toxicity tests were performed against locusta migratoria manilensis, and the results demonstrated that trypsin-treated sporulated cultures and crystal proteins had high toxicity to larval and adult locusts. cry toxin of bth-13 was detected on the midguts of treated locusts using immunofluorescent technology, which confirmed the site of action of the crystal proteins in their toxicity for locusts. (c) 2008 published by elsevier inc. |
| WOS关键词 | INSECTICIDAL CRYSTAL PROTEINS ; IDENTIFICATION ; LEPIDOPTERA ; MANAGEMENT ; LARVAE ; GENES ; PESTS ; FIELD ; CORN |
| WOS研究方向 | Zoology |
| WOS类目 | Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000256463300006 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375492 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Gao, Meiying |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Hubei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Lingli,Gao, Meiying,Dai, Shunying,et al. Specific activity of a bacillus thuringiensis strain against locusta migratoria manilensis[J]. Journal of invertebrate pathology,2008,98(2):169-176. |
| APA | Song, Lingli,Gao, Meiying,Dai, Shunying,Wu, Yan,Yi, Dan,&Li, Rongsen.(2008).Specific activity of a bacillus thuringiensis strain against locusta migratoria manilensis.Journal of invertebrate pathology,98(2),169-176. |
| MLA | Song, Lingli,et al."Specific activity of a bacillus thuringiensis strain against locusta migratoria manilensis".Journal of invertebrate pathology 98.2(2008):169-176. |
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来源：武汉病毒研究所
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