Establishment of an indicator cell line to quantify bovine foamy virus infection
文献类型：期刊论文
| 作者 | Ma, Zhe1,2; Hao, Peng1,2; Yao, Xue1,2; Liu, Chang1,2; Tan, Juan1,2; Liu, Li2; Yang, Rongge3; Geng, Yunqi1,2; Chen, Qimin1,2; Qiao, Wentao1,2 |
| 刊名 | Journal of basic microbiology
 |
| 出版日期 | 2008-08-01 |
| 卷号 | 48期号:4页码:278-283 |
| 关键词 | Bovine foamy virus (bfv) Bfv indicator cell lines (bicl) Infection |
| ISSN号 | 0233-111X |
| DOI | 10.1002/jobm.200700295 |
| 通讯作者 | Chen, qimin() |
| 英文摘要 | A cell line derived from baby hamster kidney (bhk-21) cells was transfected with the enhanced green fluorescent protein gene driven by the bovine foamy virus (bfv) long terminal repeat (ltr) to establish a bfv indicator cell line (bicl). among 48 clones, one clone was chosen for its little constitutive enhanced green fluorescent protein (egfp) expression and high level of egfp expression after activation by bfv infection. by detecting the egfp expression of the bfv` indicator cell line, the titers of bfv were quantified by the end point method. as a result, the titer determined by the egfp based assay 5-6 days post infection (d.p.i.) was 100 fold higher than traditional assays measuring cytopathic effects 8-9 d.p.i.. moreover, the egfp based assay was also used to determine the titer of those cells infected by bfv without inducing cytopathic effects. using this simple and rapid assay, we examined the in vitro host range of bfv. it was found that bfv can productively infect various cell lines derived from bovine, human, rat and monkey. |
| WOS关键词 | HUMAN-IMMUNODEFICIENCY-VIRUS ; LTR PROMOTER ACTIVITY ; HERPES-SIMPLEX-VIRUS ; QUANTITATIVE ASSAY ; GRAVES-DISEASE ; GENE ; DNA ; PROTEIN ; SPUMAVIRUSES ; GENOME |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000259044400007 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375503 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Qimin |
| 作者单位 | 1.Nankai Univ, Coll Life Sci, Key Lab Mol Microbiol & Biotechnol, Minist Educ, Tianjin 300071, Peoples R China 2.Nankai Univ, Coll Life Sci, Key Lab Funct Genom Tianjin, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Zhe,Hao, Peng,Yao, Xue,et al. Establishment of an indicator cell line to quantify bovine foamy virus infection[J]. Journal of basic microbiology,2008,48(4):278-283. |
| APA | Ma, Zhe.,Hao, Peng.,Yao, Xue.,Liu, Chang.,Tan, Juan.,...&Qiao, Wentao.(2008).Establishment of an indicator cell line to quantify bovine foamy virus infection.Journal of basic microbiology,48(4),278-283. |
| MLA | Ma, Zhe,et al."Establishment of an indicator cell line to quantify bovine foamy virus infection".Journal of basic microbiology 48.4(2008):278-283. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。