Investigation of the cyt gene in bacillus thuringiensis and the biological activities of bt isolates from the soil of china
文献类型：期刊论文
| 作者 | Wu, Yan; Gao, Meiying; Dai, Shunying; Yi, Dan; Fan, Hongyu |
| 刊名 | Biological control
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 47期号:3页码:335-339 |
| 关键词 | Bacillus thuringiensis Cyt gene Cytolytic activity Hemolytic activity |
| ISSN号 | 1049-9644 |
| DOI | 10.1016/j.biocontrol.2008.08.020 |
| 通讯作者 | Gao, meiying(mygao@wh.iov.cn) |
| 英文摘要 | The presence of cyt genes was investigated in 80 type strains of bacillus thuringiensis and 143 isolates obtained from soil samples of china by pcr amplification using two pairs of primers for the cyt1 and cyt2 genes. three type strains of serotypes h11ac, h14 and h36, eight isolates belonging to h3, h14, h18 and h21, and one isolate of unknown serotype harbored cyt genes. we also tested the cytolytic activity for mammal cells, the hemolytic activity for sheep erythrocytes and insecticidal activity against mosquitoes of five isolates that contained cyt genes but did not belong to b. thuringiensis serovar israelensis. the protein profiles of the five isolates were different from those of the type strains of b. thuringiensis serovar israelensis, and among the five isolates, only y-5 showed mosquitocidal activity against larvae of culex quinquefasciatus. all five of the isolates exhibited hemolytic activity, but only three could cause the cell death of a549 cells. the cytopathological changes induced by nx-4 in some a549 cells were characterized with cell-ballooning. (c) 2008 elsevier inc. all rights reserved. |
| WOS关键词 | CULEX-QUINQUEFASCIATUS DIPTERA ; PESTICIDAL CRYSTAL PROTEINS ; SUBSP ISRAELENSIS ; PARASPORAL INCLUSIONS ; DIVERSITY ; TOXICITY ; STRAINS ; IDENTIFICATION ; CULICIDAE ; INSECT |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Entomology |
| WOS类目 | Biotechnology & Applied Microbiology ; Entomology |
| 语种 | 英语 |
| WOS记录号 | WOS:000261262600011 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375507 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Gao, Meiying |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Hubei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Yan,Gao, Meiying,Dai, Shunying,et al. Investigation of the cyt gene in bacillus thuringiensis and the biological activities of bt isolates from the soil of china[J]. Biological control,2008,47(3):335-339. |
| APA | Wu, Yan,Gao, Meiying,Dai, Shunying,Yi, Dan,&Fan, Hongyu.(2008).Investigation of the cyt gene in bacillus thuringiensis and the biological activities of bt isolates from the soil of china.Biological control,47(3),335-339. |
| MLA | Wu, Yan,et al."Investigation of the cyt gene in bacillus thuringiensis and the biological activities of bt isolates from the soil of china".Biological control 47.3(2008):335-339. |
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来源：武汉病毒研究所
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