Simultaneous detection of norovirus and rotavirus in oysters by multiplex rt-pcr
文献类型：期刊论文
| 作者 | Kou, Xiaoxia1,2; Wu, Qingping1; Wang, Dapeng1,2,3; Zhang, Jumei1 |
| 刊名 | Food control
 |
| 出版日期 | 2008-07-01 |
| 卷号 | 19期号:7页码:722-726 |
| 关键词 | Norovirus Rotavirus Multiplex rt-pcr Oyster |
| ISSN号 | 0956-7135 |
| DOI | 10.1016/j.foodcont.2007.07.001 |
| 通讯作者 | Wu, qingping(dikangzhang@yahoo.com.cn) |
| 英文摘要 | Multiplex reverse transcription-polymerase chain reaction (rt-pcr) was used to detect noroviruses (nvs) and rotaviruses (rvs) in artificially and naturally contaminated oysters. clearly expected amplicons of 327 bp from nvs and 392 bp from rvs were obtained, respectively. the sensitivity was 200 pg rna/g oyster tissue. in a total of 150 naturally contaminated oysters that were highly polluted by feces, 5 (3.33%) were rotavirus, 21 (14%) were norovirus gii and 6 (4%) were norovirus gi. the results clearly demonstrate that the multiplex rt-pcr method is a specific, sensitive and time-saving method and can be used to detect nvs and rvs in marine products before their entering the markets. (c) 2007 elsevier ltd. all rights reserved. |
| WOS关键词 | POLYMERASE-CHAIN-REACTION ; HEPATITIS-A VIRUS ; REVERSE TRANSCRIPTION ; REACTION AMPLIFICATION ; STOOL SAMPLES ; SHELLFISH ; GASTROENTERITIS ; ASTROVIRUS ; PATHOGENS ; OUTBREAKS |
| WOS研究方向 | Food Science & Technology |
| WOS类目 | Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000253607800011 |
| 出版者 | ELSEVIER SCI LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375509 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wu, Qingping |
| 作者单位 | 1.Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, Guangzhou 510070, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 3.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kou, Xiaoxia,Wu, Qingping,Wang, Dapeng,et al. Simultaneous detection of norovirus and rotavirus in oysters by multiplex rt-pcr[J]. Food control,2008,19(7):722-726. |
| APA | Kou, Xiaoxia,Wu, Qingping,Wang, Dapeng,&Zhang, Jumei.(2008).Simultaneous detection of norovirus and rotavirus in oysters by multiplex rt-pcr.Food control,19(7),722-726. |
| MLA | Kou, Xiaoxia,et al."Simultaneous detection of norovirus and rotavirus in oysters by multiplex rt-pcr".Food control 19.7(2008):722-726. |
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来源：武汉病毒研究所
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