Production and characterization of monoclonal antibodies to nucleoprotein of marburg virus
文献类型：期刊论文
| 作者 | Zhang Jibin1; Lu Xiumei1; Wei Hongping2; Cheng Longyan2; He Jin1; Zhang Zhiping2; Zhang Xianen2; Yu Ziniu1 |
| 刊名 | Hybridoma
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 27期号:6页码:423-429 |
| ISSN号 | 1554-0014 |
| DOI | 10.1089/hyb.2008.0044 |
| 通讯作者 | Yu ziniu(yz41@mail.hzau.edu.cn) |
| 英文摘要 | Monoclonal antibodies (mabs) against the nucleoprotein of marburg virus (marv-np) with high specificity and activity would be useful for the diagnosis of marv. in this report, a recombinant marv-np was successfully expressed by an escherichia coli expression system. after immunization and cell fusion, three mouse hybridomas (1h4, 2g1, and 3b5) producing mabs to marv-np were established. the mabs obtained were fully characterized using indirect elisa and western blot analysis. the elisa results showed that the mabs' titers were in the range of 1: 6.400 x 10(3) - 1:1.280 x 10(4) in culture fluids, and 1:1.024 x 10(6) - 1:8.192 x 10(6) in ascitic fluids. the isotypes of the three mabs were tested to be igg1k and all the mabs recognized the same antigenic epitope. western blot analyses demonstrated that all the mabs were directed against marv-np with the affinity constants (kaff) of about 1.100 x 10(9) m(-1), 1.235 x 10(9) m(-1), and 1.408 x 10(9) m(-1) for the mabs 1h4, 2g1, and 3b5, respectively. |
| WOS关键词 | LINKED-IMMUNOSORBENT-ASSAY ; EBOLA |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000261990800002 |
| 出版者 | MARY ANN LIEBERT INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375510 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yu Ziniu |
| 作者单位 | 1.Huazhong Agr Univ, Natl Engn Res Ctr Microbial Pesticides, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Jibin,Lu Xiumei,Wei Hongping,et al. Production and characterization of monoclonal antibodies to nucleoprotein of marburg virus[J]. Hybridoma,2008,27(6):423-429. |
| APA | Zhang Jibin.,Lu Xiumei.,Wei Hongping.,Cheng Longyan.,He Jin.,...&Yu Ziniu.(2008).Production and characterization of monoclonal antibodies to nucleoprotein of marburg virus.Hybridoma,27(6),423-429. |
| MLA | Zhang Jibin,et al."Production and characterization of monoclonal antibodies to nucleoprotein of marburg virus".Hybridoma 27.6(2008):423-429. |
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来源：武汉病毒研究所
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