Vaccination of mice with recombinant baculovirus expressing spike or nucleocapsid protein of sars-like coronavirus generates humoral and cellular immune responses
文献类型：期刊论文
| 作者 | Bai, Bingke1,2; Lu, Xinya1,2; Meng, Jin1,2; Hu, Qinxue1; Mao, Panyong3; Lu, Baojing1,2; Chen, Ze1; Yuan, Zhiming1; Wang, Hanzhong1 |
| 刊名 | Molecular immunology
 |
| 出版日期 | 2008-02-01 |
| 卷号 | 45期号:4页码:868-875 |
| 关键词 | Cellular immune response Humoral immune response Recombinant baculovirus Sars-like coronavirus |
| ISSN号 | 0161-5890 |
| DOI | 10.1016/j.molimm.2007.08.010 |
| 通讯作者 | Wang, hanzhong(wanghz@wh.iov.cn) |
| 英文摘要 | Continuous efforts have been made to develop a prophylactic vaccine against severe acute respiratory syndrome coronavirus (sars-cov). in this study, two recombinant baculoviruses, vac-n and vac-s, were constructed, which contained the mammalian-cell activate promoter element, human elongation factor la-subunit (ef-1 alpha), the human cytomegalovirus (cmv) immediate-early promoter, and the nucleocapsid (n) or spike (s) gene of bat sars-like cov (sl-cov) under the control of the cmv promoter. mice were subcutaneously and intraperitoneally injected with recombinant baculovirus, and both humoral and cellular immune responses were induced in the vaccinated groups. the secretion level of ifn-gamma was much higher than that of il-4 in vac-n or vac-s immunized groups, suggesting a strong th 1 bias towards cellular immune responses. additionally, a marked increase of cd4 t cell immune responses and high levels of anti-sars-cov immoral responses were also detected in the vac-n or vac-s immunized groups. in contrast, there were significantly weaker cellular immune responses, as well as less antibody production than in the control groups. our data demonstrates that the recombinant baculovirus can serve as an effective vaccine strategy. in addition, because effective sars vaccines should act to not only prevent the reemergence of sars-cov, but also to provide cross-protection against sl-cov, findings in this study may have implications for developing such cross-protective vaccines. (c) 2007 elsevier ltd. all rights reserved. |
| WOS关键词 | ACUTE-RESPIRATORY-SYNDROME ; EFFICIENT GENE-TRANSFER ; MAMMALIAN-CELLS ; INSECT CELLS ; DNA VACCINE ; INFECTIOUS-BRONCHITIS ; STRUCTURAL PROTEINS ; PROTECTIVE IMMUNITY ; MEDIATED EXPRESSION ; ANTIBODY-RESPONSES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Immunology |
| WOS类目 | Biochemistry & Molecular Biology ; Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000252232400002 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375511 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Hanzhong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.302 Hosp, Inst Infect Dis, Dept Virol, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Bingke,Lu, Xinya,Meng, Jin,et al. Vaccination of mice with recombinant baculovirus expressing spike or nucleocapsid protein of sars-like coronavirus generates humoral and cellular immune responses[J]. Molecular immunology,2008,45(4):868-875. |
| APA | Bai, Bingke.,Lu, Xinya.,Meng, Jin.,Hu, Qinxue.,Mao, Panyong.,...&Wang, Hanzhong.(2008).Vaccination of mice with recombinant baculovirus expressing spike or nucleocapsid protein of sars-like coronavirus generates humoral and cellular immune responses.Molecular immunology,45(4),868-875. |
| MLA | Bai, Bingke,et al."Vaccination of mice with recombinant baculovirus expressing spike or nucleocapsid protein of sars-like coronavirus generates humoral and cellular immune responses".Molecular immunology 45.4(2008):868-875. |
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来源：武汉病毒研究所
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