Difference in receptor usage between severe acute respiratory syndrome (sars) coronavirus and sars-like coronavirus of bat origin
文献类型：期刊论文
| 作者 | Ren, Wuze3; Qu, Xiuxia4; Li, Wendong3; Han, Zhenggang3; Yu, Meng1,2; Zhou, Peng3; Zhang, Shu-Yi5; Wang, Lin-Fa1,2; Deng, Hongkui4; Shi, Zhengli3 |
| 刊名 | Journal of virology
 |
| 出版日期 | 2008-02-01 |
| 卷号 | 82期号:4页码:1899-1907 |
| ISSN号 | 0022-538X |
| DOI | 10.1128/jvi.01085-07 |
| 通讯作者 | Wang, lin-fa(linfa.wang@csiro.au) |
| 英文摘要 | Severe acute respiratory syndrome (sars) is caused by the sars-associated coronavirus (sars-cov), which uses angiotensin-converting enzyme 2 (ace2) as its receptor for cell entry. a group of sars-like covs (sl-covs) has been identified in horseshoe bats. sl-covs and sars-covs share identical genome organizations and high sequence identities, with the main exception of the n terminus of the spike protein (s), known to be responsible for receptor binding in covs. in this study, we investigated the receptor usage of the sl-cov s by combining a human immunodeficiency virus-based pseudovirus system with cell lines expressing the ace2 molecules of human, civet, or horseshoe bat. in addition to full-length s of sl-cov and sars-cov, a series of s chimeras was constructed by inserting different sequences of the sars-cov s into the sl-cov s backbone. several important observations were made from this study. first, the sl-cov s was unable to use any of the three ace2 molecules as its receptor. second, the sars-cov s failed to enter cells expressing the bat ace2. third, the chimeric s covering the previously defined receptor-binding domain gained its ability to enter cells via human ace2, albeit with different efficiencies for different constructs. fourth, a minimal insert region (amino acids 310 to 518) was found to be sufficient to convert the sl-cov s from non-ace2 binding to human ace2 binding, indicating that the sl-cov s is largely compatible with sars-cov s protein both in structure and in function. the significance of these findings in relation to virus origin, virus recombination, and host switching is discussed. |
| WOS关键词 | INFECTIOUS-BRONCHITIS VIRUS ; ANGIOTENSIN-CONVERTING ENZYME-2 ; SPIKE PROTEIN ; FUNCTIONAL-CHARACTERIZATION ; NEUTRALIZING ANTIBODIES ; NATURAL RECOMBINATION ; HORSESHOE BATS ; VARIABLE RATES ; IDENTIFICATION ; GLYCOPROTEIN |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000252909300025 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375517 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Lin-Fa |
| 作者单位 | 1.CSIRO Livestock Ind, Australian Anim Hlth Lab, Geelong, Vic 3220, Australia 2.Australian Biosecur Cooperat Res Ctr Emerging Inf, Geelong, Vic 3220, Australia 3.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan, Peoples R China 4.Peking Univ, Coll Life Sci, Minist Educ, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China 5.E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ren, Wuze,Qu, Xiuxia,Li, Wendong,et al. Difference in receptor usage between severe acute respiratory syndrome (sars) coronavirus and sars-like coronavirus of bat origin[J]. Journal of virology,2008,82(4):1899-1907. |
| APA | Ren, Wuze.,Qu, Xiuxia.,Li, Wendong.,Han, Zhenggang.,Yu, Meng.,...&Shi, Zhengli.(2008).Difference in receptor usage between severe acute respiratory syndrome (sars) coronavirus and sars-like coronavirus of bat origin.Journal of virology,82(4),1899-1907. |
| MLA | Ren, Wuze,et al."Difference in receptor usage between severe acute respiratory syndrome (sars) coronavirus and sars-like coronavirus of bat origin".Journal of virology 82.4(2008):1899-1907. |
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来源：武汉病毒研究所
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