Subnanometer-resolution structures of the grass carp reovirus core and virion
文献类型：期刊论文
| 作者 | Cheng, Lingpeng1,2; Fang, Qin1,3; Shah, Sanket2; Atanasov, Ivo C.1; Zhou, Z. Hong1,2 |
| 刊名 | Journal of molecular biology
 |
| 出版日期 | 2008-09-26 |
| 卷号 | 382期号:1页码:213-222 |
| 关键词 | Grass carp reovirus Aquareovirus Cryo-electron microscopy 3-d structure Reoviridae |
| ISSN号 | 0022-2836 |
| DOI | 10.1016/j.jmb.2008.06.075 |
| 通讯作者 | Fang, qin(qfang@whi.iov.cn) |
| 英文摘要 | Grass carp reovirus (gcrv) is a member of the aquareovirus genius of the family reoviridae, a large family of double-stranded rna (dsrna) viruses infecting plants, insects, fishes and mammals. we report the first subnanometer-resolution three-dimensional structures of both gcrv core and virion by cryoelectron microscopy. these structures have allowed the delineation of interactions among the over 1000 molecules in this enormous macromolecular machine and a detailed comparison with other dsrna viruses at the secondary-structure level. the gcrv core structure shows that the inner proteins have strong structural similarities with those of orthoreoviruses even at the level of secondary-structure elements, indicating that the structures involved in viral dsrna interaction and transcription are highly conserved. in contrast, the level of similarity in structures decreases in the proteins situated in the outer layers of the virion. the proteins involved in host recognition and attachment exhibit the least similarities to other members of reoviridae. furthermore, in gcrv, the rna-translocating turrets are in an open state and lack a counterpart for the sigma 1 protein situated on top of the close turrets observed in mammalian orthoreovirus. interestingly, the distribution and the organization of gcrv core proteins resemble those of the cytoplasmic polyhedrosis virus, a cypovirus and the structurally simplest member of the reoviridae family. our results suggest that gcrv occupies a unique structure niche between the simpler cypoviruses and the considerably more complex mammalian orthoreovirus, thus providing an important model for understanding the structural and functional conservation and diversity of this enormous family of dsrna viruses. (c) 2008 elsevier ltd. all rights reserved. |
| WOS关键词 | CYTOPLASMIC POLYHEDROSIS-VIRUS ; SINGLE-PARTICLE RECONSTRUCTIONS ; PROTEIN-RNA INTERACTIONS ; CRYOELECTRON MICROSCOPY ; ELECTRON CRYOMICROSCOPY ; IMAGE-RECONSTRUCTION ; 3-DIMENSIONAL RECONSTRUCTION ; ANGSTROM RESOLUTION ; GENUS AQUAREOVIRUS ; FAMILY REOVIRIDAE |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000259588500018 |
| 出版者 | ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375519 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Fang, Qin |
| 作者单位 | 1.Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genent, Calif Nanosyst Inst, Los Angeles, CA 90095 USA 2.Univ Texas Houston, Sch Med, Dept Pathol & Lab Med, Houston, TX 77030 USA 3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, Lingpeng,Fang, Qin,Shah, Sanket,et al. Subnanometer-resolution structures of the grass carp reovirus core and virion[J]. Journal of molecular biology,2008,382(1):213-222. |
| APA | Cheng, Lingpeng,Fang, Qin,Shah, Sanket,Atanasov, Ivo C.,&Zhou, Z. Hong.(2008).Subnanometer-resolution structures of the grass carp reovirus core and virion.Journal of molecular biology,382(1),213-222. |
| MLA | Cheng, Lingpeng,et al."Subnanometer-resolution structures of the grass carp reovirus core and virion".Journal of molecular biology 382.1(2008):213-222. |
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来源：武汉病毒研究所
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