Pathogenicity and immunogenicity of recombinant tiantan vaccinia virus with deleted c12l and a53r genes
文献类型：期刊论文
| 作者 | Dai, Kaifan1,2,3; Liu, Ying1; Liu, Mingjie1; Xu, Jianqing1; Huang, Wei1; Huang, Xianggang1; Liu, Lianxing1,2,3; Wan, Yanmin1; Hao, Yanling1; Shao, Yiming1,2 |
| 刊名 | Vaccine
 |
| 出版日期 | 2008-09-15 |
| 卷号 | 26期号:39页码:5062-5071 |
| 关键词 | Hiv vaccine Replicating tiantan vaccinia virus Pathogenicity and immunogenicity Cytokine viroceptor genes |
| ISSN号 | 0264-410X |
| DOI | 10.1016/j.vaccine.2008.06.011 |
| 通讯作者 | Shao, yiming(yshao@bbn.cn) |
| 英文摘要 | Interest is increasing regarding replicating poxvirus as hiv vaccine vector. in china, the tiantan vaccinia virus (tv) has been used most extensively in the battle of eradicating smallpox. recently, tv was developing as vaccine vector to fight against infectious diseases such as human immunodeficiency virus (hiv). however, replicating vaccinia virus sometimes may pose serious post-vaccination complications, especially in immunosuppressed individuals. to develop a safer and more effective tv-based vector, we constructed c12l (vil-18 binding protein) and a53r (vtnf receptor homolog) gene-deleted mutants which are based on parental tv and vtkgpe (tv expressing hiv gagpol and env gene), respectively. the pathogenicity and immunogenicity were also evaluated. deleting these two immunomodulatory genes lessened the virulence of the parental virus in both mice and rabbit models. notably, c12l deletion mutant attenuated the skin virulence of parental virus by as high as approximate 2 logs. furthermore, vtkgpe with a53r and c12l gene deletion retains the high immunogenicity of the parental virus to elicit strong humoral and cellular responses to the hiv target genes despite the remarkable attenuation. these data suggest that deletion of the cytokine viroceptor gene is feasible to obtain a safer and replication-competent tv vector for vaccination and immunotherapy. (c) 2008 elsevier ltd. all rights reserved. |
| WOS关键词 | NECROSIS-FACTOR RECEPTOR ; IMMUNE-RESPONSES ; INFLAMMATORY RESPONSE ; T-CELLS ; IN-VIVO ; VIRULENCE ; PROTEIN ; POXVIRUSES ; ENCODES ; VECTORS |
| WOS研究方向 | Immunology ; Research & Experimental Medicine |
| WOS类目 | Immunology ; Medicine, Research & Experimental |
| 语种 | 英语 |
| WOS记录号 | WOS:000259936400012 |
| 出版者 | ELSEVIER SCI LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375521 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Shao, Yiming |
| 作者单位 | 1.China CDC, Natl Ctr AIDS STD Control & Prevent NCAIDS, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Natl Key Lab Virol, Beijing, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, Kaifan,Liu, Ying,Liu, Mingjie,et al. Pathogenicity and immunogenicity of recombinant tiantan vaccinia virus with deleted c12l and a53r genes[J]. Vaccine,2008,26(39):5062-5071. |
| APA | Dai, Kaifan.,Liu, Ying.,Liu, Mingjie.,Xu, Jianqing.,Huang, Wei.,...&Shao, Yiming.(2008).Pathogenicity and immunogenicity of recombinant tiantan vaccinia virus with deleted c12l and a53r genes.Vaccine,26(39),5062-5071. |
| MLA | Dai, Kaifan,et al."Pathogenicity and immunogenicity of recombinant tiantan vaccinia virus with deleted c12l and a53r genes".Vaccine 26.39(2008):5062-5071. |
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来源：武汉病毒研究所
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