Genetic disruption of kshv major latent nuclear antigen lana enhances viral lytic transcriptional program
文献类型：期刊论文
| 作者 | Li, Qiuhua1,3; Zhou, Fuchun1,2; Ye, Fengchun1,2; Gao, Shou-Jiang1,2,3,4,5,6,7 |
| 刊名 | Virology
 |
| 出版日期 | 2008-09-30 |
| 卷号 | 379期号:2页码:234-244 |
| 关键词 | Kshv Lana Viral latency Lytic replication Vital transcriptional program Reverse genetics |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2008.06.043 |
| 通讯作者 | Gao, shou-jiang(gaos@uthscsa.edu) |
| 英文摘要 | Following primary infection, kshv establishes a lifelong persistent latent infection in the host. the mechanism of kshv latency is not fully understood. the latent nuclear antigen (lana or lna) encoded by orf73 is one of a few vital genes expressed during kshv latency, and is consistently detected in all kshv-related malignancies. lana is essential for kshv episome persistence, and regulates the expression of viral lytic genes through epigenetic silencing, and inhibition of the expression and transactivation function of the key kshv lytic replication initiator rta (orf50). in this study, we used a genetic approach to examine the role of lana in regulating kshv lytic replication program. deletion of lana did not affect the expression of its adjacent genes vcyclin (orf72) and vflip (orf71). in contrast, the expression levels of viral lytic genes including immediate-early gene rta, early genes mta (orf57), vil-6 (orf-k2) and orf59, and late gene orf-k8.1 were increased before and after vital lytic induction with 12-o-tetradecanoyl-phorbol-13-acetate and sodium butyrate. this enhanced expression of vital lytic genes was also observed following overexpression of rta with or without simultaneous chemical induction. consistent with these results, the lana mutant cells produced more infectious virions than the wild-type virus cells did, furthermore, genetic repair of the mutant virus reverted the phenotypes to those of wild-type virus. together, these results have demonstrated that, in the context of vital genome, lana contributes to kshv latency by regulating the expression of rta and its downstream genes. (c) 2008 elsevier inc. all rights reserved. |
| WOS关键词 | SARCOMA-ASSOCIATED HERPESVIRUS ; BACTERIAL ARTIFICIAL CHROMOSOME ; PROTEIN-KINASE PATHWAYS ; KAPOSIS-SARCOMA ; ENDOTHELIAL-CELLS ; PRIMARY INFECTION ; EPISOME PERSISTENCE ; DNA-REPLICATION ; HUMAN-HERPESVIRUS-8 ; EXPRESSION |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000259549500008 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375525 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Gao, Shou-Jiang |
| 作者单位 | 1.Univ Texas Hlth Sci Ctr San Antonio, Tumor Virol Program, Greehey Childrens Canc Res Inst, San Antonio, TX 78229 USA 2.Univ Texas Hlth Sci Ctr San Antonio, Dept Pediat, San Antonio, TX 78229 USA 3.Univ Texas Hlth Sci Ctr San Antonio, Dept Microbiol & Immunol, San Antonio, TX 78229 USA 4.Univ Texas Hlth Sci Ctr San Antonio, Dept Med, San Antonio, TX 78229 USA 5.Univ Texas Hlth Sci Ctr San Antonio, Dept Mol Med, San Antonio, TX 78229 USA 6.Univ Texas Hlth Sci Ctr San Antonio, Canc Therapy & Res Ctr, San Antonio, TX 78229 USA 7.Chinese Acad Sci, Tumor Virol Grp, Wuhan Inst Virol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Qiuhua,Zhou, Fuchun,Ye, Fengchun,et al. Genetic disruption of kshv major latent nuclear antigen lana enhances viral lytic transcriptional program[J]. Virology,2008,379(2):234-244. |
| APA | Li, Qiuhua,Zhou, Fuchun,Ye, Fengchun,&Gao, Shou-Jiang.(2008).Genetic disruption of kshv major latent nuclear antigen lana enhances viral lytic transcriptional program.Virology,379(2),234-244. |
| MLA | Li, Qiuhua,et al."Genetic disruption of kshv major latent nuclear antigen lana enhances viral lytic transcriptional program".Virology 379.2(2008):234-244. |
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来源：武汉病毒研究所
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