Inhibition of reverse transcriptase activity of hepatitis b virus polymerase by beta-l-w4a-tp
文献类型：期刊论文
| 作者 | Wang, X. Y.1; Gao, L. L.1; Hu, Z. H.2; Wang, H. L.2; Deng, F.2; Lin, J. S.1 |
| 刊名 | Acta virologica
 |
| 出版日期 | 2008 |
| 卷号 | 52期号:1页码:47-52 |
| 关键词 | Hepatitis b virus Polymerase Reverse transcriptase Beta-l-d4a-tp |
| ISSN号 | 0001-723X |
| 通讯作者 | Lin, j. s.(jslin2001@163.com) |
| 英文摘要 | Beta-l-enantiomer of 2',3'-didehydro-2',3'-dideoxyadenosine-5'-triphosphate (beta-l-d4a-tp) has previously been proven to inhibit the replication of viral dna in the hep g2 2.2.15 cells and in transgenic mouse harboring 1.3-fold-overlength genomic of hepatitis b virus (hbv). to study the inhibition mechanism of the nucleoside analog beta-l-d4a-tp, a polymerase reaction in vitro with the recombinant hbv nucleocapsids was conducted to determine the exact mode of inhibition of the hbv replication by beta-l-d4a-tp. the hbv viral dna and viral dna-polymerase complex formed in the polymerase reaction were assayed. the results of this study showed that beta-l-d4a-tp inhibited the replication of hbv dna by inactivating the reverse transcriptase (rt) activity in a concentration-dependent manner. the kinetics of beta-l-d4a-tp inhibition of the rt activity was the result of an apparent competitive inhibition with datp. |
| WOS关键词 | NUCLEOSIDE ANALOGS ; DNA-SYNTHESIS ; INTRACELLULAR METABOLISM ; CAPSID PROTEIN ; REPLICATION ; DOMAIN ; ENCAPSIDATION ; RESISTANCE ; BIOLOGY ; AGENTS |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000260060800006 |
| 出版者 | AEPRESS SRO |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375526 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Lin, J. S. |
| 作者单位 | 1.Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Inst Liver Dis, Wuhan 430030, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430030, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, X. Y.,Gao, L. L.,Hu, Z. H.,et al. Inhibition of reverse transcriptase activity of hepatitis b virus polymerase by beta-l-w4a-tp[J]. Acta virologica,2008,52(1):47-52. |
| APA | Wang, X. Y.,Gao, L. L.,Hu, Z. H.,Wang, H. L.,Deng, F.,&Lin, J. S..(2008).Inhibition of reverse transcriptase activity of hepatitis b virus polymerase by beta-l-w4a-tp.Acta virologica,52(1),47-52. |
| MLA | Wang, X. Y.,et al."Inhibition of reverse transcriptase activity of hepatitis b virus polymerase by beta-l-w4a-tp".Acta virologica 52.1(2008):47-52. |
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来源：武汉病毒研究所
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