中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Inhibition of reverse transcriptase activity of hepatitis b virus polymerase by beta-l-w4a-tp

文献类型:期刊论文

作者Wang, X. Y.1; Gao, L. L.1; Hu, Z. H.2; Wang, H. L.2; Deng, F.2; Lin, J. S.1
刊名Acta virologica
出版日期2008
卷号52期号:1页码:47-52
关键词Hepatitis b virus Polymerase Reverse transcriptase Beta-l-d4a-tp
ISSN号0001-723X
通讯作者Lin, j. s.(jslin2001@163.com)
英文摘要Beta-l-enantiomer of 2',3'-didehydro-2',3'-dideoxyadenosine-5'-triphosphate (beta-l-d4a-tp) has previously been proven to inhibit the replication of viral dna in the hep g2 2.2.15 cells and in transgenic mouse harboring 1.3-fold-overlength genomic of hepatitis b virus (hbv). to study the inhibition mechanism of the nucleoside analog beta-l-d4a-tp, a polymerase reaction in vitro with the recombinant hbv nucleocapsids was conducted to determine the exact mode of inhibition of the hbv replication by beta-l-d4a-tp. the hbv viral dna and viral dna-polymerase complex formed in the polymerase reaction were assayed. the results of this study showed that beta-l-d4a-tp inhibited the replication of hbv dna by inactivating the reverse transcriptase (rt) activity in a concentration-dependent manner. the kinetics of beta-l-d4a-tp inhibition of the rt activity was the result of an apparent competitive inhibition with datp.
WOS关键词NUCLEOSIDE ANALOGS ; DNA-SYNTHESIS ; INTRACELLULAR METABOLISM ; CAPSID PROTEIN ; REPLICATION ; DOMAIN ; ENCAPSIDATION ; RESISTANCE ; BIOLOGY ; AGENTS
WOS研究方向Virology
WOS类目Virology
语种英语
WOS记录号WOS:000260060800006
出版者AEPRESS SRO
URI标识http://www.irgrid.ac.cn/handle/1471x/2375526
专题武汉病毒研究所
通讯作者Lin, J. S.
作者单位1.Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Inst Liver Dis, Wuhan 430030, Hubei, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430030, Hubei, Peoples R China
推荐引用方式
GB/T 7714
Wang, X. Y.,Gao, L. L.,Hu, Z. H.,et al. Inhibition of reverse transcriptase activity of hepatitis b virus polymerase by beta-l-w4a-tp[J]. Acta virologica,2008,52(1):47-52.
APA Wang, X. Y.,Gao, L. L.,Hu, Z. H.,Wang, H. L.,Deng, F.,&Lin, J. S..(2008).Inhibition of reverse transcriptase activity of hepatitis b virus polymerase by beta-l-w4a-tp.Acta virologica,52(1),47-52.
MLA Wang, X. Y.,et al."Inhibition of reverse transcriptase activity of hepatitis b virus polymerase by beta-l-w4a-tp".Acta virologica 52.1(2008):47-52.

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来源:武汉病毒研究所

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