New target tissue for food-borne virus detection in oysters
文献类型：期刊论文
| 作者 | Wang, D.1,2,3; Wu, Q.1; Yao, L.1,2,3; Wei, M.1; Kou, X.1; Zhang, J.1 |
| 刊名 | Letters in applied microbiology
 |
| 出版日期 | 2008-11-01 |
| 卷号 | 47期号:5页码:405-409 |
| 关键词 | Food-borne virus New target tissue Oyster Tissues |
| ISSN号 | 0266-8254 |
| DOI | 10.1111/j.1472-765x.2008.02445.x |
| 通讯作者 | Wu, q.(wuqp203@yahoo.com.cn) |
| 英文摘要 | Aim: to evaluate the different tissues of naturally contaminated oyster for food-borne virus detection. methods and results: the different tissues of 136 field oyster samples were analysed for norovirus (nv), hepatitis a virus (hav) and rotavirus (rv) by reverse transcription (rt)-pcr and were confirmed by sequencing. these viruses were detected in 20 samples (14.71%), showing positivity for nv (1.47%), hav (5.15%) and rv (8.82%). furthermore, among different tissues, the highest positive rate of the food-borne viruses was found in the gills (14.71%), followed by the stomach (13.97%) and the digestive diverticula (13.24%). conclusions: the food-borne viruses were detected in the gills, stomach, digestive diverticula and the cilia of the mantle. in addition, the results showed that the gills are one of the appropriate tissues for viral detection in oysters by nucleic acid assay. significance and impact of the study: this is the first paper to report on the presence of food-borne viruses in the gills and the cilia of the mantle of naturally contaminated oysters. the research team hopes that the results of the study will be of help in sampling the appropriate tissues for the detection of food-borne viruses in commercial oysters. |
| WOS关键词 | HEPATITIS-A VIRUS ; NORWALK-LIKE VIRUS ; SHELLFISH CONSUMPTION ; BIVALVE SHELLFISH ; UNITED-STATES ; RT-PCR ; GASTROENTERITIS ; PERSISTENCE ; ROTAVIRUS ; CALICIVIRUSES |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000260138700008 |
| 出版者 | BLACKWELL PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375527 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wu, Q. |
| 作者单位 | 1.Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, Guangzhou 510070, Guangdong, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Peoples R China 3.Grad Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, D.,Wu, Q.,Yao, L.,et al. New target tissue for food-borne virus detection in oysters[J]. Letters in applied microbiology,2008,47(5):405-409. |
| APA | Wang, D.,Wu, Q.,Yao, L.,Wei, M.,Kou, X.,&Zhang, J..(2008).New target tissue for food-borne virus detection in oysters.Letters in applied microbiology,47(5),405-409. |
| MLA | Wang, D.,et al."New target tissue for food-borne virus detection in oysters".Letters in applied microbiology 47.5(2008):405-409. |
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来源：武汉病毒研究所
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