Gold nanoparticle enhanced immuno-pcr for ultrasensitive detection of hantaan virus nucleocapsid protein
文献类型：期刊论文
| 作者 | Chen, Longyan1,2; Wei, Hongping1; Guo, Yongchao1; Cui, Zongqiang1; Zhang, Zhiping1; Zhang, Xian-En1 |
| 刊名 | Journal of immunological methods
 |
| 出版日期 | 2009-07-31 |
| 卷号 | 346期号:1-2页码:64-70 |
| 关键词 | Hantaan virus nucleocapsid protein Gold nanoparticle Immuno-pcr |
| ISSN号 | 0022-1759 |
| DOI | 10.1016/j.jim.2009.05.007 |
| 通讯作者 | Wei, hongping(hpwei@wh.iov.cn) |
| 英文摘要 | A functionalized gold nanoparticle (gnp) enhanced ultrasensitive immuno-pcr assay (gnp-ipcr on elisa plate), which was modified from the recent developed bio-barcode assay (bca) technique, was developed to detect hantaan virus nucleocapsid protein (hnp). during the assay, the target antigen hnp was captured by a polyclonal antibody coated on elisa microplate wells, followed by adding gnp dually modified with oligonucleotides and a hnp specific monoclonal antibody l13 (mab l13) to form a sandwich immuno-complex. the oligonucleotides on the gnp contained two strands: one as capture dna immobilized on the surface of the gnp through au-s bond and the other as signal amplification dna, which was partially complementary with the capture dna. after the immuno-complex was formed, the signal dna was released by heating, and consequently characterized by pcr/gel electrophoresis and sybr-green real time pcr. the detection limit of this method could reach down to 10 fg/ml for detecting purified hnp in buffers as well as in human serum, which was similar to 7 orders of magnitude more sensitive than that of conventional elisa. the current assay format might be adopted for other proteins that need ultra-high sensitive detection. (c) 2009 elsevier b.v. all rights reserved. |
| WOS关键词 | POLYMERASE-CHAIN-REACTION ; BARCODE AMPLIFICATION ASSAY ; HANTAVIRUS INFECTIONS ; HEMORRHAGIC-FEVER ; RENAL SYNDROME ; SEROLOGICAL ASSAYS ; SURFACE COVERAGE ; RT-PCR ; ANTIGEN ; SENSITIVITY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Immunology |
| WOS类目 | Biochemical Research Methods ; Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000268199900008 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375530 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wei, Hongping |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Longyan,Wei, Hongping,Guo, Yongchao,et al. Gold nanoparticle enhanced immuno-pcr for ultrasensitive detection of hantaan virus nucleocapsid protein[J]. Journal of immunological methods,2009,346(1-2):64-70. |
| APA | Chen, Longyan,Wei, Hongping,Guo, Yongchao,Cui, Zongqiang,Zhang, Zhiping,&Zhang, Xian-En.(2009).Gold nanoparticle enhanced immuno-pcr for ultrasensitive detection of hantaan virus nucleocapsid protein.Journal of immunological methods,346(1-2),64-70. |
| MLA | Chen, Longyan,et al."Gold nanoparticle enhanced immuno-pcr for ultrasensitive detection of hantaan virus nucleocapsid protein".Journal of immunological methods 346.1-2(2009):64-70. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。