Imaging viral behavior in mammalian cells with self-assembled capsid-quantum-dot hybrid particles
文献类型：期刊论文
| 作者 | Li, Feng1,3; Zhang, Zhi-Ping1; Peng, Jun2; Cui, Zong-Qiang1; Pang, Dai-Wen2; Li, Ke1,3; Wei, Hong-Ping1; Zhou, Ya-Feng1; Wen, Ji-Kai4; Zhang, Xian-En1 |
| 刊名 | Small
 |
| 出版日期 | 2009-03-20 |
| 卷号 | 5期号:6页码:718-726 |
| 关键词 | Hybrid materials Quantum dots Self-assembly Simian virus 40 Virus tracing |
| ISSN号 | 1613-6810 |
| DOI | 10.1002/smll.200801303 |
| 通讯作者 | Zhang, xian-en(x.zhang@wh.iov.cn) |
| 英文摘要 | Unique spectral properties of quantum dots (qds) enable ultrasensitive and long-term biolabeling. aiming to trace the infection, movement, and localization of viruses in living cells, qd-containing virus-like particles (vlps) of simian virus 40 (sv40), termed svlp-qds, are constructed by in vitro self-assembly of the major capsid protein of sv40. svlp-qds show homogeneity in size (approximate to 24 nm), similarity in spectral properties to unencapsidated qds, and considerable stability. when incubated with living cells, svlp-qds are shown to enter the cells by caveolar endocytosis, travel along the microtubules, and accumulate in the endoplasmic reticulum. this process mimics the early infection steps of sv40. this is the first paradigm of imaging viral behaviors with encapsidated qds in living cells. the method may provide a new alternative for various purposes, such as tracing viruses or viral components, targeted nanoparticle delivery, and probing of drug delivery. |
| WOS关键词 | VIRUS-LIKE PARTICLES ; ENDOPLASMIC-RETICULUM ; IN-VITRO ; CAVEOLAR ENDOCYTOSIS ; SIMIAN VIRUS-40 ; PROTEIN VP1 ; SIMIAN-VIRUS-40 ; POLYOMAVIRUS ; TRANSPORT ; MEMBRANE |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| WOS类目 | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter |
| 语种 | 英语 |
| WOS记录号 | WOS:000264934200011 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375532 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Xian-En |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 4.Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England |
| 推荐引用方式 GB/T 7714 | Li, Feng,Zhang, Zhi-Ping,Peng, Jun,et al. Imaging viral behavior in mammalian cells with self-assembled capsid-quantum-dot hybrid particles[J]. Small,2009,5(6):718-726. |
| APA | Li, Feng.,Zhang, Zhi-Ping.,Peng, Jun.,Cui, Zong-Qiang.,Pang, Dai-Wen.,...&Zhang, Xian-En.(2009).Imaging viral behavior in mammalian cells with self-assembled capsid-quantum-dot hybrid particles.Small,5(6),718-726. |
| MLA | Li, Feng,et al."Imaging viral behavior in mammalian cells with self-assembled capsid-quantum-dot hybrid particles".Small 5.6(2009):718-726. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。