Enhancing immune responses against sars-cov nucleocapsid dna vaccine by co-inoculating interleukin-2 expressing vector in mice
文献类型：期刊论文
| 作者 | Hu, Hui1,2; Tao, Ling1; Wang, Yabin2; Chen, Liying2; Yang, Jihong1,3; Wang, Hanzhong1 |
| 刊名 | Biotechnology letters
 |
| 出版日期 | 2009-11-01 |
| 卷号 | 31期号:11页码:1685-1693 |
| 关键词 | Dna vaccine Interleukin-2 gene Nucleocapsid protein Sars-cov |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-009-0061-y |
| 通讯作者 | Yang, jihong() |
| 英文摘要 | The immunogenicity of sars-cov nucleocapsid dna vaccine and the immunoregulatory activity of interleukin-2 (il-2) were investigated. dna vaccine plasmids, pcdna-n and pcdna-il2, were constructed and inoculated into balb/c mice with or without pcdna-il2 by intramuscular injection. cellular and humoral immune responses were assessed by indirect elisa, lymphocyte proliferation assays, elispot and facs. the nucleocapsid dna vaccine had good immunogenicity and can induce specific humoral and cellular immunity in balb/c mice, while il-2 plays an immunoadjuvant role and enhances specific immune responses. this study provides a frame of reference for the design of dna vaccines against sars-cov. |
| WOS关键词 | ACUTE RESPIRATORY SYNDROME ; SYNDROME CORONAVIRUS ; MOUSE MODEL ; IN-VIVO ; VIRUS ; PROTEIN ; INDUCTION ; PROLIFERATION ; IMMUNIZATION ; EFFICACY |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000270646800006 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375537 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yang, Jihong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Henan, Peoples R China 3.Cent China Normal Univ, Life Sci Coll, Wuhan 430079, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Hui,Tao, Ling,Wang, Yabin,et al. Enhancing immune responses against sars-cov nucleocapsid dna vaccine by co-inoculating interleukin-2 expressing vector in mice[J]. Biotechnology letters,2009,31(11):1685-1693. |
| APA | Hu, Hui,Tao, Ling,Wang, Yabin,Chen, Liying,Yang, Jihong,&Wang, Hanzhong.(2009).Enhancing immune responses against sars-cov nucleocapsid dna vaccine by co-inoculating interleukin-2 expressing vector in mice.Biotechnology letters,31(11),1685-1693. |
| MLA | Hu, Hui,et al."Enhancing immune responses against sars-cov nucleocapsid dna vaccine by co-inoculating interleukin-2 expressing vector in mice".Biotechnology letters 31.11(2009):1685-1693. |
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来源：武汉病毒研究所
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