Humoral and cellular immune responses induced by 3a dna vaccines against severe acute respiratory syndrome (sars) or sars-like coronavirus in mice
文献类型：期刊论文
| 作者 | Lu, Baojing1,2; Tao, Ling1,2; Wang, Ting1; Zheng, Zhenhua1,2; Li, Bao1,2; Chen, Ze1; Huang, Yi1; Hu, Qinxue1; Wang, Hanzhong1 |
| 刊名 | Clinical and vaccine immunology
 |
| 出版日期 | 2009 |
| 卷号 | 16期号:1页码:73-77 |
| ISSN号 | 1556-6811 |
| DOI | 10.1128/cvi.00261-08 |
| 通讯作者 | Hu, qinxue(huqinxue@hotmail.com) |
| 英文摘要 | Vaccine development for severe acute respiratory syndrome coronavirus (sars-cov) has mainly focused on the spike (s) protein. however, the variation of the s gene between viruses may affect the efficacy of a vaccine, particularly for cross-protection against sars-like cov (sl-cov). recently, a more conserved group-specific open reading frame (orf), the 3a gene, was found in both sars-cov and sl-cov. here, we studied the immunogenicity of human sars-cov 3a and bat sl-cov 3a dna vaccines in mice through electroporation immunization followed by enzyme-linked immunosorbent, enzyme-linked immunospot, and flow cytometry assays. our results showed that high levels of specific humoral responses were induced by sars-cov 3a and sl-cov 3a dna vaccines. furthermore, a strong th1-based cellular immune response was stimulated by both dna vaccines. the vaccines stimulated gamma interferon production mainly by cd8(+) t cells and interleukin-2 (il-2) mainly by cd4(+) t cells. of interest, the frequency of il-2-positive cells elicited by the sars-cov 3a dna vaccine was significantly higher than that elicited by the sl-cov 3a dna vaccine. in summary, our study provides a reference for designing cross-protective dna vaccines based on the group-specific orfs of covs. |
| WOS关键词 | SPIKE GLYCOPROTEIN ; EXPRESSING SPIKE ; PROTEIN ; VIRUS ; MEMORY ; NEUTRALIZATION ; IMMUNIZATION ; GENERATION ; GENOME ; CELLS |
| WOS研究方向 | Immunology ; Infectious Diseases ; Microbiology |
| WOS类目 | Immunology ; Infectious Diseases ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000262157500009 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375540 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Hu, Qinxue |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Baojing,Tao, Ling,Wang, Ting,et al. Humoral and cellular immune responses induced by 3a dna vaccines against severe acute respiratory syndrome (sars) or sars-like coronavirus in mice[J]. Clinical and vaccine immunology,2009,16(1):73-77. |
| APA | Lu, Baojing.,Tao, Ling.,Wang, Ting.,Zheng, Zhenhua.,Li, Bao.,...&Wang, Hanzhong.(2009).Humoral and cellular immune responses induced by 3a dna vaccines against severe acute respiratory syndrome (sars) or sars-like coronavirus in mice.Clinical and vaccine immunology,16(1),73-77. |
| MLA | Lu, Baojing,et al."Humoral and cellular immune responses induced by 3a dna vaccines against severe acute respiratory syndrome (sars) or sars-like coronavirus in mice".Clinical and vaccine immunology 16.1(2009):73-77. |
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来源：武汉病毒研究所
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