Expression and homology modelling of sterol 14 alpha-demethylase of magnaporthe grisea and its interaction with azoles
文献类型：期刊论文
| 作者 | Yang, Jiaoyan1; Zhang, Qingye2; Liao, Mingjun1,3; Xiao, Min1; Xiao, Wenjing2; Yang, Shao2; Wan, Nan1 |
| 刊名 | Pest management science
 |
| 出版日期 | 2009-03-01 |
| 卷号 | 65期号:3页码:260-265 |
| 关键词 | Cyp51 Magnaporthe grisea Expression Binding spectrum Homology modelling Molecular docking |
| ISSN号 | 1526-498X |
| DOI | 10.1002/ps.1680 |
| 通讯作者 | Yang, shao(yang-shao@tom.com) |
| 英文摘要 | Background: magnaporthe grisea (hebert) me barr infection is one of the most serious diseases for cultivated rice in the world. sterol 14 alpha-demethylase (cyp51) is an important drug target for microbial pathogenic infections. to exploit specific and effective fungicides for m. grisea better, the authors have analysed the characteristics of interaction between sterol 14a-demethylase from m. grisea (mgcyp51) and azoles. mgcyp51 with truncation of n-terminal residues was cloned and expressed in e. coli, difference binding spectra of mgcyp51 induced by addition of four commercial azoles were determined and molecular modelling of mgcyp51 based on the crystal structure of mycobacterium tuberculosis lehmann & newman and docking with the azoles were performed. results: the affinity of the azoles for mgcyp51 was positively correlated with their hydrophobicity. amino acid residues tyr112, phe120, phe220, his308 and phe497 of mgcyp51, forming a large hydrophobic cavity, are the key residues interacting with azole fungicides. furthermore, phe220 and phe497 are fungus and species specific respectively. conclusion: the results suggest that the more potent azole fungicides for mgcyp51 should possess more hydrophobic groups interacting with residues phe220 and phe497. (c) 2009 society of chemical industry |
| WOS关键词 | CANDIDA-ALBICANS ; ASPERGILLUS-FUMIGATUS ; ESCHERICHIA-COLI ; CYTOCHROME-P450 ; CYP51 ; ANTIFUNGALS ; INHIBITION ; BINDING ; PROTEIN ; DESIGN |
| WOS研究方向 | Agriculture ; Entomology |
| WOS类目 | Agronomy ; Entomology |
| 语种 | 英语 |
| WOS记录号 | WOS:000263777300006 |
| 出版者 | JOHN WILEY & SONS LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375542 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yang, Shao |
| 作者单位 | 1.Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China 2.Minist Educ, Key Lab Pesticide & Chem Biol CCNU, Wuhan, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Jiaoyan,Zhang, Qingye,Liao, Mingjun,et al. Expression and homology modelling of sterol 14 alpha-demethylase of magnaporthe grisea and its interaction with azoles[J]. Pest management science,2009,65(3):260-265. |
| APA | Yang, Jiaoyan.,Zhang, Qingye.,Liao, Mingjun.,Xiao, Min.,Xiao, Wenjing.,...&Wan, Nan.(2009).Expression and homology modelling of sterol 14 alpha-demethylase of magnaporthe grisea and its interaction with azoles.Pest management science,65(3),260-265. |
| MLA | Yang, Jiaoyan,et al."Expression and homology modelling of sterol 14 alpha-demethylase of magnaporthe grisea and its interaction with azoles".Pest management science 65.3(2009):260-265. |
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来源：武汉病毒研究所
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