Increasing m2 epitope density enhances systemic and mucosal immune responses to influenza a virus
文献类型：期刊论文
| 作者 | Pei, Shaobo1,2; Xiong, Ning1,2; Zhang, Yong1,2; Chen, Shiyun1 |
| 刊名 | Biotechnology letters
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 31期号:12页码:1851-1856 |
| 关键词 | Influenza M2 protein Mucosal immunity Repeating epitopes Systemic immunity |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-009-0102-6 |
| 通讯作者 | Chen, shiyun(sychen@wh.iov.cn) |
| 英文摘要 | Recombinant proteins bearing one (sm2) or three (3sm2) copies of m2 epitope from influenza virus were expressed in escherichia coli. mice were administrated with these two proteins and systemic and mucosal immune responses were analyzed. compared with sm2, 3sm2 induced m2-specific serum igg and mucosal iga antibodies with neutralizing activity more efficiently in the whole immune course and the later mucosal immunization improved this advantage. mdck cells pretreated with 3sm2 antisera showed less pathogenic morphological changes compared with that of pretreated with sm2. together, our results demonstrated that high epitope density in one recombinant protein can enhance humoral immune responses in both systemic and mucosal immunization, and combination of systemic and mucosal immunization enhances this advantage. |
| WOS关键词 | MATRIX PROTEIN-2 ; EXTRACELLULAR DOMAIN ; MICE ; VACCINE ; ECTODOMAIN ; ANTIBODIES ; INFECTION ; H5N1 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000271481200005 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375543 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Shiyun |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei Province, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pei, Shaobo,Xiong, Ning,Zhang, Yong,et al. Increasing m2 epitope density enhances systemic and mucosal immune responses to influenza a virus[J]. Biotechnology letters,2009,31(12):1851-1856. |
| APA | Pei, Shaobo,Xiong, Ning,Zhang, Yong,&Chen, Shiyun.(2009).Increasing m2 epitope density enhances systemic and mucosal immune responses to influenza a virus.Biotechnology letters,31(12),1851-1856. |
| MLA | Pei, Shaobo,et al."Increasing m2 epitope density enhances systemic and mucosal immune responses to influenza a virus".Biotechnology letters 31.12(2009):1851-1856. |
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来源：武汉病毒研究所
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