Angiotensin-converting enzyme 2 (ace2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirus
文献类型：期刊论文
| 作者 | Xu, Lili1; Zhang, Yanfang1; Liu, Yun1; Chen, Zhiwei2; Deng, Hongkui3; Ma, Zhongbin1; Wang, Hualin1; Hu, Zhihong1; Deng, Fei1 |
| 刊名 | Journal of general virology
 |
| 出版日期 | 2009-11-01 |
| 卷号 | 90页码:2695-2703 |
| ISSN号 | 0022-1317 |
| DOI | 10.1099/vir.0.013490-0 |
| 通讯作者 | Deng, fei(df@wh.iov.cn) |
| 英文摘要 | Raccoon dog is one of the suspected intermediate hosts of severe acute respiratory syndrome coronavirus (sars-cov). in this study, the angiotensin-converting enzyme 2 (ace2) gene of raccoon dog (rdace2) was cloned and sequenced. the amino acid sequence of rdace2 has identities of 99.3, 89.2, 83.9 and 80.4% to ace2 proteins from dog, masked palm civet (pcace2), human (huace2) and bat, respectively. there are six amino acid changes in rdace2 compared with huace2, and four changes compared with pcace2, within the 18 residues of ace2 known to make direct contact with the sars-cov s protein. a hela cell line stably expressing rdace2 was established; western blot analyses and an enzyme-activity assay indicated that the cell line expressed ace2 at a similar level to two previously established cell lines that express ace2 from human and masked palm civet, respectively. human immunodeficiency virus-backboned pseudoviruses expressing spike proteins derived from human sars-cov or sars-cov-like viruses of masked palm civets and raccoon dogs were tested for their entry efficiency into these cell lines. the results showed that rdace2 is a more efficient receptor for human sars-cov, but not for sars-cov-like viruses of masked palm civets and raccoon dogs, than huace2 or pcace2. this study provides useful data to elucidate the role of raccoon dog in sars outbreaks. |
| WOS关键词 | SARS-CORONAVIRUS ; SEQUENCE ALIGNMENT ; BINDING DOMAIN ; PALM CIVETS ; IDENTIFICATION ; VIRUS ; BATS ; REPLICATION ; EVOLUTION ; CHINA |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000271440500015 |
| 出版者 | SOC GENERAL MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375545 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Deng, Fei |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Univ Hong Kong, Li Ka Shing Fac Med, AIDS Inst, Hong Kong, Hong Kong, Peoples R China 3.Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Lili,Zhang, Yanfang,Liu, Yun,et al. Angiotensin-converting enzyme 2 (ace2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirus[J]. Journal of general virology,2009,90:2695-2703. |
| APA | Xu, Lili.,Zhang, Yanfang.,Liu, Yun.,Chen, Zhiwei.,Deng, Hongkui.,...&Deng, Fei.(2009).Angiotensin-converting enzyme 2 (ace2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirus.Journal of general virology,90,2695-2703. |
| MLA | Xu, Lili,et al."Angiotensin-converting enzyme 2 (ace2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirus".Journal of general virology 90(2009):2695-2703. |
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来源：武汉病毒研究所
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