Ompr positively regulates urease expression to enhance acid survival of yersinia pseudotuberculosis
文献类型：期刊论文
| 作者 | Hu, Yangbo1,3; Lu, Pei1,3; Wang, Yao1; Ding, Lisha1,3; Atkinson, Steve2; Chen, Shiyun1 |
| 刊名 | Microbiology-sgm
 |
| 出版日期 | 2009-08-01 |
| 卷号 | 155页码:2522-2531 |
| ISSN号 | 1350-0872 |
| DOI | 10.1099/mic.0.028381-0 |
| 通讯作者 | Chen, shiyun(sychen@wh.iov.cn) |
| 英文摘要 | Yersinia pseudotuberculosis is an enteric bacterium which must overcome the acidic stress in host organs for successful colonization, but how this bacterium survives in acidic conditions remains largely unknown. in the present study, the importance of ompr in acid survival of y. pseudo tuberculosis ypiii was confirmed by the fact that mutation of ompr (strain delta ompr) greatly reduced cell survival at ph 4.5 or lower. to characterize the regulatory role of ompr in this acid survival process, proteomic analysis was carried out to compare ypiii at ph 7.0 and ph 4.5 with delta ompr at ph 7.0, and urease components were revealed to be the main targets for ompr regulation. addition of urea to the culture medium also enhanced acid survival of ypiii but not delta ompr and urease activity was significantly induced by acid in ypiii but not in delta ompr. each of the seven components of the ypiii urease gene cluster was fused to a lacz reporter and their expression was dramatically decreased in a delta ompr background; this supports the notion that ompr positively regulates urease expression. furthermore, gel shift analysis revealed that ompr binds to the deduced promoter regions of three polycistronic transcriptional units (ureabc, ureef and uregd) in the urease cluster, suggesting that the regulation of ompr to urease components is direct. taken together, these data strongly suggest that ompr activates urease expression to enhance acid survival in y. pseudotuberculosis. |
| WOS关键词 | ENTERICA SEROVAR TYPHIMURIUM ; ESCHERICHIA-COLI ; HELICOBACTER-PYLORI ; SALMONELLA-TYPHIMURIUM ; TOLERANCE RESPONSE ; LOW-PH ; TRANSCRIPTIONAL ACTIVATOR ; 2-COMPONENT SYSTEM ; RESISTANCE SYSTEM ; IN-VITRO |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000269028900007 |
| 出版者 | SOC GENERAL MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375546 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Shiyun |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Univ Nottingham, Ctr Biomol Sci, Inst Infect Immun & Inflammat, Nottingham NG7 2UH, England 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Yangbo,Lu, Pei,Wang, Yao,et al. Ompr positively regulates urease expression to enhance acid survival of yersinia pseudotuberculosis[J]. Microbiology-sgm,2009,155:2522-2531. |
| APA | Hu, Yangbo,Lu, Pei,Wang, Yao,Ding, Lisha,Atkinson, Steve,&Chen, Shiyun.(2009).Ompr positively regulates urease expression to enhance acid survival of yersinia pseudotuberculosis.Microbiology-sgm,155,2522-2531. |
| MLA | Hu, Yangbo,et al."Ompr positively regulates urease expression to enhance acid survival of yersinia pseudotuberculosis".Microbiology-sgm 155(2009):2522-2531. |
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来源：武汉病毒研究所
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