Hbx protein induces expression of mig and increases migration of leukocytes through activation of nf-kappa b
文献类型：期刊论文
| 作者 | Xia, Li-Min1; Huang, Wen-Jie2,3; Wu, Jian-Guo4; Yang, Yong-Bo4; Zhang, Qiong1; Zhou, Zhen-Zhen1; Zhu, Hui-Fen2; Lei, Pin2; Shen, Guan-Xin2; Tian, De-An1 |
| 刊名 | Virology
 |
| 出版日期 | 2009-03-15 |
| 卷号 | 385期号:2页码:335-342 |
| 关键词 | Hbv Mig Hbx Nf-kappa b Promoter Migration |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2008.11.042 |
| 通讯作者 | Tian, de-an(datian@tjh.tjmu.edu.cn) |
| 英文摘要 | Elevated expression of monokine induced by the interferon-gamma (mig) has been shown in hbv carriers, and it is involved in the infiltration of inflammatory cells and liver damage after hbv infection. however, the molecular mechanisms by which hbv-induced mig expression have not been characterized. our results indicated that hbx protein induced mig expression in a dose-dependent manner. such increase was due to the direct binding of nf-kappa b to the mig promoter. by luciferase, chromatin immunoprecipitation and electrophoretic mobility shift assays, we demonstrated that the nf-kappa b binding site at positions - 147 was essential for transcriptional activation of mic promoter by hbx protein. chemotaxis assay showed that the up-regulation of mig protein levels enhanced the migration of peripheral blood lymphocytes (pbls), and inhibition of nf-kappa b significantly decreased the chemotaxis activity. our findings provide a new insight into how leukocytes migrate to liver, and disclose a new regulatory mechanism of mig expression after hbv infection. (c) 2008 published by elsevier inc. |
| WOS关键词 | CYTOTOXIC T-LYMPHOCYTES ; INTERFERON-INDUCIBLE PROTEIN-10 ; VIRUS-X PROTEIN ; IN-VIVO ; CHEMOKINE ; CELLS ; TRANSCRIPTION ; GAMMA ; LIVER ; GENE |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000264252200006 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375548 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Tian, De-An |
| 作者单位 | 1.Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Div Gastroenterol, Wuhan 430030, Hubei Province, Peoples R China 2.Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Immunol, Wuhan 430030, Hubei Province, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 4.Wuhan Univ, Coll Live Sci, Wuhan 430072, Hubei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Li-Min,Huang, Wen-Jie,Wu, Jian-Guo,et al. Hbx protein induces expression of mig and increases migration of leukocytes through activation of nf-kappa b[J]. Virology,2009,385(2):335-342. |
| APA | Xia, Li-Min.,Huang, Wen-Jie.,Wu, Jian-Guo.,Yang, Yong-Bo.,Zhang, Qiong.,...&Tian, De-An.(2009).Hbx protein induces expression of mig and increases migration of leukocytes through activation of nf-kappa b.Virology,385(2),335-342. |
| MLA | Xia, Li-Min,et al."Hbx protein induces expression of mig and increases migration of leukocytes through activation of nf-kappa b".Virology 385.2(2009):335-342. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。