Comparative analysis of the immunogenicity of sars-cov nucleocapsid dna vaccine administrated with different routes in mouse model
文献类型：期刊论文
| 作者 | Hu, Hui1,2; Huang, Xianqing2; Tao, Ling1; Huang, Yi1; Cui, Bao-an1,2; Wang, Hanzhong1 |
| 刊名 | Vaccine
 |
| 出版日期 | 2009-03-10 |
| 卷号 | 27期号:11页码:1758-1763 |
| 关键词 | Sars-cov Nucleocapsid protein Dna vaccine Immunization routes |
| ISSN号 | 0264-410X |
| DOI | 10.1016/j.vaccine.2009.01.021 |
| 通讯作者 | Cui, bao-an() |
| 英文摘要 | The development of strategies to augment the immunogenicity of dna vaccines is critical for improving their clinical utility. one such strategy involves using the different immune routes with dna vaccines. in the present study, the immunogenicity of sars-cov nucleocapsid dna vaccine, induced by using the current routine vaccination routes (intramuscularly, by electroporation, or orally using live-attenuated salmonella typhimurium), was compared in mouse model. the comparison between the three vaccination routes indicated that immunization intramuscularly induced a moderate t cell response and antibody response. mice administrated by electroporation induced the highest antibody response among the three immunization groups and a mid-level of cellular response. in contrast, the orally dna vaccine evoked vigorous t cell response and a weak antibody production. these results indicated that the distinct types of immune responses were generated by the different routes of dna immunization. in addition, our results also show that the delivery of dna vaccines by electroporation and orally using live-attenuated salmonella in vivo is an effective method to increase the immune responses. further studies could be carried out using a combination strategy of both oral and electroporation immunizations to stimulate higher cellular and humoral immune responses. (c) 2009 elsevier ltd. all rights reserved. |
| WOS关键词 | ACUTE RESPIRATORY SYNDROME ; ATTENUATED SALMONELLA-TYPHIMURIUM ; IN-VIVO ELECTROPORATION ; SYNDROME CORONAVIRUS ; ANTIBODY-RESPONSES ; IMMUNE-RESPONSES ; HEPATITIS-VIRUS ; POTENTIAL USE ; PROTEIN ; MICE |
| WOS研究方向 | Immunology ; Research & Experimental Medicine |
| WOS类目 | Immunology ; Medicine, Research & Experimental |
| 语种 | 英语 |
| WOS记录号 | WOS:000264619900015 |
| 出版者 | ELSEVIER SCI LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375549 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Cui, Bao-an |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Hui,Huang, Xianqing,Tao, Ling,et al. Comparative analysis of the immunogenicity of sars-cov nucleocapsid dna vaccine administrated with different routes in mouse model[J]. Vaccine,2009,27(11):1758-1763. |
| APA | Hu, Hui,Huang, Xianqing,Tao, Ling,Huang, Yi,Cui, Bao-an,&Wang, Hanzhong.(2009).Comparative analysis of the immunogenicity of sars-cov nucleocapsid dna vaccine administrated with different routes in mouse model.Vaccine,27(11),1758-1763. |
| MLA | Hu, Hui,et al."Comparative analysis of the immunogenicity of sars-cov nucleocapsid dna vaccine administrated with different routes in mouse model".Vaccine 27.11(2009):1758-1763. |
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来源：武汉病毒研究所
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