Comparing the ability of a series of viral protein-expressing plasmid dnas to protect against h5n1 influenza virus
文献类型：期刊论文
| 作者 | Chen, Quanjiao1,2; Kuang, Haimen3; Wang, Huadong1; Fang, Fang3; Yang, Zhongdong4; Zhang, Zhiping1; Zhang, Xianen1; Chen, Ze1,3,4 |
| 刊名 | Virus genes
 |
| 出版日期 | 2009-02-01 |
| 卷号 | 38期号:1页码:30-38 |
| 关键词 | H5n1 Hemagglutinin Neuraminidase Matrix protein Nucleoprotein Dna vaccine |
| ISSN号 | 0920-8569 |
| DOI | 10.1007/s11262-008-0305-2 |
| 通讯作者 | Chen, ze(chenze2005@263.net) |
| 英文摘要 | Avian influenza has been regarded as a human health threat. a major measure to prevent its outbreak is vaccination. in this study, a series of expression plasmids carrying the hemagglutinin (ha), neuraminidase (na), nucleoprotein (np), matrix protein 1 (m1), and matrix protein 2 (m2) genes, respectively, of the avian influenza virus (aiv) a/chicken/henan/12/2004(h5n1) strain were constructed. these plasmids were administered to mice by electroporation (50 mu g for each per administration, 1-5 times, at an interval of 2 weeks), and the mice were challenged with the homologous virus later. the mice immunized with ha plasmid once and the na plasmid twice survived 100%, and those with np plasmid showed 60-80% survival rate with at least three immunizations. the mice immunized with m1 plasmid survived 25% with five immunizations, while m2 plasmid had no protection even with five immunizations. the mixture of m1 and np plasmids protected 95% of the mice against the homologous virus, and 80% of the mice against a challenge with heterologous h1n1 (pr8) virus. moreover, the homologous protection lasted at least 6 months. our data provided a basis for selecting multiple-component aiv vaccines. |
| WOS关键词 | A VIRUS ; BALB/C MICE ; IN-VIVO ; NUCLEOPROTEIN ; VACCINE ; IMMUNIZATION ; INFECTION ; IMMUNITY ; HEMAGGLUTININ ; GENE |
| WOS研究方向 | Genetics & Heredity ; Virology |
| WOS类目 | Genetics & Heredity ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000262409100004 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375552 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Ze |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China 4.Shanghai Inst Biol Prod, Shanghai 200052, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Quanjiao,Kuang, Haimen,Wang, Huadong,et al. Comparing the ability of a series of viral protein-expressing plasmid dnas to protect against h5n1 influenza virus[J]. Virus genes,2009,38(1):30-38. |
| APA | Chen, Quanjiao.,Kuang, Haimen.,Wang, Huadong.,Fang, Fang.,Yang, Zhongdong.,...&Chen, Ze.(2009).Comparing the ability of a series of viral protein-expressing plasmid dnas to protect against h5n1 influenza virus.Virus genes,38(1),30-38. |
| MLA | Chen, Quanjiao,et al."Comparing the ability of a series of viral protein-expressing plasmid dnas to protect against h5n1 influenza virus".Virus genes 38.1(2009):30-38. |
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来源：武汉病毒研究所
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