Heterologous expression of human paraoxonases in pseudomonas aeruginosa inhibits biofilm formation and decreases antibiotic resistance
文献类型：期刊论文
| 作者 | Ma, Fang1,2; Wang, Yao1; Zhang, Yong1,2; Xiong, Ning1,2; Yang, Baoyu1; Chen, Shiyun1 |
| 刊名 | Applied microbiology and biotechnology
 |
| 出版日期 | 2009-05-01 |
| 卷号 | 83期号:1页码:135-141 |
| 关键词 | Pseudomonas aeruginosa Biofilm Antibiotic resistance Human paraoxonases Quorum sensing |
| ISSN号 | 0175-7598 |
| DOI | 10.1007/s00253-009-1890-2 |
| 通讯作者 | Chen, shiyun(sychen@wh.iov.cn) |
| 英文摘要 | Quorum sensing (qs) regulates virulence and biofilm formation in pseudomonas aeruginosa and other medically relevant bacteria. human paraoxonases (hpons) are a family of closely related enzymes with multiple functions, including inactivation of the qs signal molecule in p. aeruginosa. however, there is no direct evidence to show the functions of hpons on biofilm formation and antibiotic resistance in p. aeruginosa. in the present study, hpons (hpon1, hpon2, and hpon3) genes were respectively cloned into the pmekm12 shuttle vector and transformed into p. aeruginosa strain pao1. expression of the three recombinant proteins was confirmed by western blotting, and growth of the recombinant strains was not affected by the hpons gene expression. biofilm formation and antibiotics resistance of the hpons recombinant strains were analyzed. our results showed that biofilm formation was significantly inhibited in all of the three hpons recombinant strains. interestingly, this inhibition can be reverted by addition of the corresponding hpons polyclonal antibodies in the culture media, further indicating that the inhibition of biofilm formation was due to hpons protein expression. in addition, we also demonstrated that hpons expression decreased resistance of p. aeruginosa to gentamicin and ceftazidima, two antibiotics clinically used for the treatment of p. aeruginosa infection. |
| WOS关键词 | HOMOSERINE LACTONE ACYLASE ; QUORUM-SENSING MOLECULES ; MESORHIZOBIUM-HUAKUII ; MICROBIAL BIOFILMS ; BACTERIAL BIOFILMS ; GENE ; PON1 ; INACTIVATION ; DEGRADATION ; DEFICIENCY |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000265382300014 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375556 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Shiyun |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Fang,Wang, Yao,Zhang, Yong,et al. Heterologous expression of human paraoxonases in pseudomonas aeruginosa inhibits biofilm formation and decreases antibiotic resistance[J]. Applied microbiology and biotechnology,2009,83(1):135-141. |
| APA | Ma, Fang,Wang, Yao,Zhang, Yong,Xiong, Ning,Yang, Baoyu,&Chen, Shiyun.(2009).Heterologous expression of human paraoxonases in pseudomonas aeruginosa inhibits biofilm formation and decreases antibiotic resistance.Applied microbiology and biotechnology,83(1),135-141. |
| MLA | Ma, Fang,et al."Heterologous expression of human paraoxonases in pseudomonas aeruginosa inhibits biofilm formation and decreases antibiotic resistance".Applied microbiology and biotechnology 83.1(2009):135-141. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。