Positive regulation of flhdc expression by ompr in yersinia pseudotuberculosis
文献类型：期刊论文
| 作者 | Hu, Yangbo1,3; Wang, Yao1; Ding, Lisha1,3; Lu, Pei1,3; Atkinson, Steve2; Chen, Shiyun1 |
| 刊名 | Microbiology-sgm
 |
| 出版日期 | 2009-11-01 |
| 卷号 | 155页码:3622-3631 |
| ISSN号 | 1350-0872 |
| DOI | 10.1099/mic.0.030908-0 |
| 通讯作者 | Chen, shiyun(sychen@wh.iov.cn) |
| 英文摘要 | Ompr has been demonstrated to negatively regulate the expression of the flagellar master operon flhdc in a wide variety of bacterial species. here we report the positive regulation of flhdc expression by ompr in yersinia pseudotuberculosis. a sigma(70)-dependent promoter was identified by primer extension analysis and an active region with two conserved ompr-binding sites around the flhdc promoter was confirmed. to confirm the regulation of flhdc expression by ompr, flhdc as well as the downstream flagellar genes flia, flgd, flga, flgm, flic and flaa were fused to lacz, and decreased expression of all these genes in an ompr mutant (delta ompr) was detected. furthermore, delta ompr was defective in bacterial motility and flagella synthesis. this defect was due to the low level of expression of flhdc in delta ompr since overproduction of flhdc in delta ompr restored bacterial motility. the importance of two conserved ompr-binding sites around the flhdc promoter region in the regulation of flhdc expression by ompr was demonstrated by the fact that mutation of either one or both sites significantly decreased the promoter activity in the wild-type but not in delta ompr. the binding of ompr to these two sites was also demonstrated by dna mobility shift assay. the possible mechanism underlying this positive regulation in y. pseudotuberculosis is discussed. to our knowledge, this is the first report to demonstrate that ompr positively regulates flhdc expression. |
| WOS关键词 | ENTEROHEMORRHAGIC ESCHERICHIA-COLI ; GRAM-NEGATIVE BACTERIA ; H-NS PROTEIN ; MASTER OPERON ; SALMONELLA-TYPHIMURIUM ; FLAGELLAR EXPRESSION ; TRANSCRIPTION FACTOR ; SWIMMING MOTILITY ; BIOFILM FORMATION ; ACETYL PHOSPHATE |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000272121300017 |
| 出版者 | SOC GENERAL MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375558 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Shiyun |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Univ Nottingham, Inst Infect Immun & Inflammat, Ctr Biomol Sci, Nottingham NG7 2UH, England 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Yangbo,Wang, Yao,Ding, Lisha,et al. Positive regulation of flhdc expression by ompr in yersinia pseudotuberculosis[J]. Microbiology-sgm,2009,155:3622-3631. |
| APA | Hu, Yangbo,Wang, Yao,Ding, Lisha,Lu, Pei,Atkinson, Steve,&Chen, Shiyun.(2009).Positive regulation of flhdc expression by ompr in yersinia pseudotuberculosis.Microbiology-sgm,155,3622-3631. |
| MLA | Hu, Yangbo,et al."Positive regulation of flhdc expression by ompr in yersinia pseudotuberculosis".Microbiology-sgm 155(2009):3622-3631. |
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来源：武汉病毒研究所
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