A single-amino acid substitution in west nile virus 2k peptide between ns4a and ns4b confers resistance to lycorine, a flavivirus inhibitor
文献类型：期刊论文
| 作者 | Zou, Gang1,2,3; Puig-Basagoiti, Francesc1; Zhang, Bo1; Qing, Min1,2,3; Chen, Liqiang4; Pankiewicz, Krzysztof W.4; Felczak, Krzysztof4; Yuan, Zhiming2; Shi, Pei-Yong1,5 |
| 刊名 | Virology
 |
| 出版日期 | 2009-02-05 |
| 卷号 | 384期号:1页码:242-252 |
| 关键词 | Flavivirus replication Antiviral West nile virus Flavivirus 2k peptide Viral resistance |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2008.11.003 |
| 通讯作者 | Shi, pei-yong(pei_yong.shi@novartis.com) |
| 英文摘要 | Lycorine potently inhibits flaviviruses in cell culture. at 1.2-mu m concentration, lycorine reduced viral titers of west nile (wnv), dengue, and yellow fever viruses by 10(2)- to 10(4)-fold. however, the compound did not inhibit an alphavirus (western equine encephalitis virus) or a rhabdovirus (vesicular stomatitis virus), indicating a selective antiviral spectrum. the compound exerts its antiviral activity mainly through suppression of viral rna replication. a val -> met substitution at the 9th amino acid position of the viral 2k peptide (spanning the endoplasmic reticulum membrane between ns4a and ns4b proteins) confers wnv resistance to lycorine, through enhancement of viral rna replication. initial chemistry synthesis demonstrated that modifications of the two hydroxyl groups of lycorine can increase the compound's potency, while reducing its cytotoxicity. taken together, the results have established lycorine as a flavivirus inhibitor for antiviral development. the lycorine-resistance results demonstrate a direct role of the 2k peptide in flavivirus rna synthesis. (c) 2008 elsevier inc. all rights reserved. |
| WOS关键词 | RNA-STIMULATED NTPASE ; DENGUE VIRUS ; POLYMERASE NS5 ; PROTEIN ; INTERFERON ; REPLICATION ; KUNJIN ; IDENTIFICATION ; AMARYLLIDACEAE ; EXPRESSION |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000263039200030 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375563 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Shi, Pei-Yong |
| 作者单位 | 1.New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12237 USA 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Beijing 100864, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China 4.Univ Minnesota, Ctr Drug Design, Minneapolis, MN USA 5.SUNY Albany, Sch Publ Hlth, Dept Biomed Sci, New York, NY USA |
| 推荐引用方式 GB/T 7714 | Zou, Gang,Puig-Basagoiti, Francesc,Zhang, Bo,et al. A single-amino acid substitution in west nile virus 2k peptide between ns4a and ns4b confers resistance to lycorine, a flavivirus inhibitor[J]. Virology,2009,384(1):242-252. |
| APA | Zou, Gang.,Puig-Basagoiti, Francesc.,Zhang, Bo.,Qing, Min.,Chen, Liqiang.,...&Shi, Pei-Yong.(2009).A single-amino acid substitution in west nile virus 2k peptide between ns4a and ns4b confers resistance to lycorine, a flavivirus inhibitor.Virology,384(1),242-252. |
| MLA | Zou, Gang,et al."A single-amino acid substitution in west nile virus 2k peptide between ns4a and ns4b confers resistance to lycorine, a flavivirus inhibitor".Virology 384.1(2009):242-252. |
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来源：武汉病毒研究所
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