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Chinese Academy of Sciences Institutional Repositories Grid
A transposable class i composite transposon carrying mph (methyl parathion hydrolase) from pseudomonas sp strain wbc-3

文献类型:期刊论文

作者Wei, Min1,2; Zhang, Jun-Jie1; Liu, Hong1; Wang, Shu-Jun1; Fu, He1,2; Zhou, Ning-Yi1
刊名Fems microbiology letters
出版日期2009-03-01
卷号292期号:1页码:85-91
关键词Is6100 Methyl parathion hydrolase Pseudomonas Transposon
ISSN号0378-1097
DOI10.1111/j.1574-6968.2008.01468.x
通讯作者Zhou, ning-yi(n.zhou@pentium.whiov.ac.cn)
英文摘要Pseudomonas sp. strain wbc-3 utilizes methyl parathion (o,o-dimethyl o-p-nitrophenol phosphorothioate) or para-nitrophenol as the sole source of carbon, nitrogen and energy. a gene encoding methyl parathion hydrolase (mph) had been characterized previously and found to be located on a typical class i composite transposon that comprised is6100 (tnmph). in this study, the transposability of this transposon was confirmed by transposition assays in two distinct mating-out systems. tnmph was demonstrated to transpose efficiently in a random manner in pseudomonas putida paw340 by southern blot and in ralstonia sp. u2 by sequence analysis of the tnmph insertion sites, both exhibiting mph activity. the linkage of the mph-like gene with is6100, together with the transposability of tnmph, as well as its capability to transpose in other phylogenetically divergent bacterial species, suggest that tnmph may contribute to the wide distribution of mph-like genes and the adaptation of bacteria to organophosphorus compounds.
WOS关键词TOL PLASMID PWWO ; GENETIC-ANALYSIS ; ESCHERICHIA-COLI ; OPD GENE ; ELEMENT ; PUTIDA ; SITE ; IDENTIFICATION ; MYCOBACTERIA ; STREPTOMYCES
WOS研究方向Microbiology
WOS类目Microbiology
语种英语
WOS记录号WOS:000263036900013
出版者WILEY-BLACKWELL PUBLISHING, INC
URI标识http://www.irgrid.ac.cn/handle/1471x/2375564
专题武汉病毒研究所
通讯作者Zhou, Ning-Yi
作者单位1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Wei, Min,Zhang, Jun-Jie,Liu, Hong,et al. A transposable class i composite transposon carrying mph (methyl parathion hydrolase) from pseudomonas sp strain wbc-3[J]. Fems microbiology letters,2009,292(1):85-91.
APA Wei, Min,Zhang, Jun-Jie,Liu, Hong,Wang, Shu-Jun,Fu, He,&Zhou, Ning-Yi.(2009).A transposable class i composite transposon carrying mph (methyl parathion hydrolase) from pseudomonas sp strain wbc-3.Fems microbiology letters,292(1),85-91.
MLA Wei, Min,et al."A transposable class i composite transposon carrying mph (methyl parathion hydrolase) from pseudomonas sp strain wbc-3".Fems microbiology letters 292.1(2009):85-91.

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来源:武汉病毒研究所

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