A transposable class i composite transposon carrying mph (methyl parathion hydrolase) from pseudomonas sp strain wbc-3
文献类型:期刊论文
作者 | Wei, Min1,2; Zhang, Jun-Jie1; Liu, Hong1; Wang, Shu-Jun1; Fu, He1,2; Zhou, Ning-Yi1 |
刊名 | Fems microbiology letters
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出版日期 | 2009-03-01 |
卷号 | 292期号:1页码:85-91 |
关键词 | Is6100 Methyl parathion hydrolase Pseudomonas Transposon |
ISSN号 | 0378-1097 |
DOI | 10.1111/j.1574-6968.2008.01468.x |
通讯作者 | Zhou, ning-yi(n.zhou@pentium.whiov.ac.cn) |
英文摘要 | Pseudomonas sp. strain wbc-3 utilizes methyl parathion (o,o-dimethyl o-p-nitrophenol phosphorothioate) or para-nitrophenol as the sole source of carbon, nitrogen and energy. a gene encoding methyl parathion hydrolase (mph) had been characterized previously and found to be located on a typical class i composite transposon that comprised is6100 (tnmph). in this study, the transposability of this transposon was confirmed by transposition assays in two distinct mating-out systems. tnmph was demonstrated to transpose efficiently in a random manner in pseudomonas putida paw340 by southern blot and in ralstonia sp. u2 by sequence analysis of the tnmph insertion sites, both exhibiting mph activity. the linkage of the mph-like gene with is6100, together with the transposability of tnmph, as well as its capability to transpose in other phylogenetically divergent bacterial species, suggest that tnmph may contribute to the wide distribution of mph-like genes and the adaptation of bacteria to organophosphorus compounds. |
WOS关键词 | TOL PLASMID PWWO ; GENETIC-ANALYSIS ; ESCHERICHIA-COLI ; OPD GENE ; ELEMENT ; PUTIDA ; SITE ; IDENTIFICATION ; MYCOBACTERIA ; STREPTOMYCES |
WOS研究方向 | Microbiology |
WOS类目 | Microbiology |
语种 | 英语 |
WOS记录号 | WOS:000263036900013 |
出版者 | WILEY-BLACKWELL PUBLISHING, INC |
URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375564 |
专题 | 武汉病毒研究所 |
通讯作者 | Zhou, Ning-Yi |
作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Wei, Min,Zhang, Jun-Jie,Liu, Hong,et al. A transposable class i composite transposon carrying mph (methyl parathion hydrolase) from pseudomonas sp strain wbc-3[J]. Fems microbiology letters,2009,292(1):85-91. |
APA | Wei, Min,Zhang, Jun-Jie,Liu, Hong,Wang, Shu-Jun,Fu, He,&Zhou, Ning-Yi.(2009).A transposable class i composite transposon carrying mph (methyl parathion hydrolase) from pseudomonas sp strain wbc-3.Fems microbiology letters,292(1),85-91. |
MLA | Wei, Min,et al."A transposable class i composite transposon carrying mph (methyl parathion hydrolase) from pseudomonas sp strain wbc-3".Fems microbiology letters 292.1(2009):85-91. |
入库方式: iSwitch采集
来源:武汉病毒研究所
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