Compensatory mutations in ns3 and ns5a proteins enhance the virus production capability of hepatitis c reporter virus
文献类型：期刊论文
| 作者 | Han, Qingxia1,2; Xu, Chunlian1,2; Wu, Chunchen1; Zhu, Wenbo1,2; Yang, Rongge1; Chen, Xinwen1 |
| 刊名 | Virus research
 |
| 出版日期 | 2009-10-01 |
| 卷号 | 145期号:1页码:63-73 |
| 关键词 | Hepatitis c virus Hcv Reporter virus Adaptive mutation Assembly |
| ISSN号 | 0168-1702 |
| DOI | 10.1016/j.virusres.2009.06.005 |
| 通讯作者 | Chen, xinwen(chenxw@pentium.whiov.ac.cn) |
| 英文摘要 | In this study, an infectious hcv monocistronic reporter virus was constructed by inserting an egfp gene into the c-terminus of ns5a in the jfh-1 genome. a robust adaptive mutant, which could produce infectious virions as robustly as the jfh-1 wild type in huh7.5.1 cells, was subsequently isolated by monitoring egfp fluorescence. full genomic sequencing revealed five amino acid substitutions, three located in the helicase domain of ns3 and two positioned in the c-terminus of ns5a. reverse genetics studies suggested that the ns3 and ns5a mutations acted synergistically to enhance virus production capability possibly by accelerating the virion assembly efficiency but did not affect the replication competence of the adaptive reporter virus. further analysis revealed that the m260k and t462i substitutions in ns3 and ns5a, respectively, were the key mutations. these adaptive mutations were also effective in the context of the jfh-1 genome. (c) 2009 elsevier b.v. all rights reserved. |
| WOS关键词 | CULTURE-ADAPTIVE MUTATIONS ; VIRAL-RNA REPLICATION ; INFECTION IN-VITRO ; CELL-CULTURE ; NONSTRUCTURAL PROTEIN ; FLUORESCENT PROTEIN ; PARTICLES ; HELICASE ; GENOME ; VISUALIZATION |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000270531600009 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375568 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Xinwen |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 10039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Qingxia,Xu, Chunlian,Wu, Chunchen,et al. Compensatory mutations in ns3 and ns5a proteins enhance the virus production capability of hepatitis c reporter virus[J]. Virus research,2009,145(1):63-73. |
| APA | Han, Qingxia,Xu, Chunlian,Wu, Chunchen,Zhu, Wenbo,Yang, Rongge,&Chen, Xinwen.(2009).Compensatory mutations in ns3 and ns5a proteins enhance the virus production capability of hepatitis c reporter virus.Virus research,145(1),63-73. |
| MLA | Han, Qingxia,et al."Compensatory mutations in ns3 and ns5a proteins enhance the virus production capability of hepatitis c reporter virus".Virus research 145.1(2009):63-73. |
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来源：武汉病毒研究所
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