A sequence-independent in vitro transposon-based strategy for efficient cloning of genomes of large dna viruses as bacterial artificial chromosomes
文献类型：期刊论文
| 作者 | Zhou, Fuchun1,2; Li, Qiuhua1,3; Gao, Shou-Jiang1,2,3,4,5,6,7,8 |
| 刊名 | Nucleic acids research
 |
| 出版日期 | 2009 |
| 卷号 | 37期号:1页码:8 |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gkn890 |
| 通讯作者 | Gao, shou-jiang(gaos@uthscsa.edu) |
| 英文摘要 | Bacterial artificial chromosomes (bacs) derived from genomes of large dna viruses are powerful tools for functional delineation of viral genes. current methods for cloning the genomes of large dna viruses as bacs require prior knowledge of the viral sequences or the cloning of viral dna fragments, and are tedious because of the laborious process of multiple plaque purifications, which is not feasible for some fastidious viruses. here, we describe a novel method for cloning the genomes of large dna viruses as bacs, which entails direct in vitro transposition of viral genomes with a bac cassette, and subsequent recovery in escherichia coli. determination of insertion sites and adjacent viral sequences identify the bac clones for genetic manipulation and functional characterization. compared to existing methods, this new approach is highly efficient, and does not require any information on viral sequences or cloning of viral dna fragments, and plaque purifications. this method could potentially be used for discovering previously unidentified viruses. |
| WOS关键词 | SARCOMA-ASSOCIATED HERPESVIRUS ; MURINE GAMMAHERPESVIRUS 68 ; ESCHERICHIA-COLI ; KAPOSIS-SARCOMA ; CYTOMEGALOVIRUS GENOME ; INFECTIOUS VIRUS ; CONSTRUCTION ; MUTAGENESIS ; IDENTIFICATION ; RECONSTITUTION |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000262335700028 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375570 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Gao, Shou-Jiang |
| 作者单位 | 1.Univ Texas Hlth Sci Ctr San Antonio, Greehey Childrens Canc Res Inst, Tumor Virol Program, San Antonio, TX 78229 USA 2.Univ Texas Hlth Sci Ctr San Antonio, Dept Pediat, San Antonio, TX 78229 USA 3.Univ Texas Hlth Sci Ctr San Antonio, Dept Microbiol & Immunol, San Antonio, TX 78229 USA 4.Univ Texas Hlth Sci Ctr San Antonio, Dept Mol Med, San Antonio, TX 78229 USA 5.Univ Texas Hlth Sci Ctr San Antonio, Dept Med, San Antonio, TX 78229 USA 6.Univ Texas Hlth Sci Ctr San Antonio, Dept Canc Therapy, San Antonio, TX 78229 USA 7.Univ Texas Hlth Sci Ctr San Antonio, Res Ctr, San Antonio, TX 78229 USA 8.Chinese Acad Sci, Wuhan Inst Virol, Tumor Virol Grp, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Fuchun,Li, Qiuhua,Gao, Shou-Jiang. A sequence-independent in vitro transposon-based strategy for efficient cloning of genomes of large dna viruses as bacterial artificial chromosomes[J]. Nucleic acids research,2009,37(1):8. |
| APA | Zhou, Fuchun,Li, Qiuhua,&Gao, Shou-Jiang.(2009).A sequence-independent in vitro transposon-based strategy for efficient cloning of genomes of large dna viruses as bacterial artificial chromosomes.Nucleic acids research,37(1),8. |
| MLA | Zhou, Fuchun,et al."A sequence-independent in vitro transposon-based strategy for efficient cloning of genomes of large dna viruses as bacterial artificial chromosomes".Nucleic acids research 37.1(2009):8. |
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来源：武汉病毒研究所
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