Immunogenicity difference between the sars coronavirus and the bat sars-like coronavirus spike (s) proteins
文献类型：期刊论文
| 作者 | Zhou, Peng; Han, Zhenggang; Wang, Lin-Fa2; Shi, Zhengli1 |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2009-09-18 |
| 卷号 | 387期号:2页码:326-329 |
| 关键词 | Sl-cov Spike protein Dna immunization Immunodominant region |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2009.07.025 |
| 通讯作者 | Shi, zhengli(zlshi@wh.iov.cn) |
| 英文摘要 | Sars-like coronavirus (sl-cov) in bats have a similar genomic organization to the human sars-cov. their cognate gene products are highly conserved with the exception of the n-terminal region of the s proteins, which have only 63-64% sequence identity. the n-terminal region of coronavirus s protein is responsible for virus-receptor interaction. in this study, the immunogenicity of the sl-cov s protein (s(sl)) was studied and compared with that of sars-cov (s(sars)). dna immunization in mice with s(sl) elicited a high titer of antibodies against hiv-pseudotyped s(sl). the sera had low cross-reactivity, but no neutralization activity, for the hiv-pseudotyped s(sars). studies using wild bat sera revealed that it is highly likely that the immunodominant epitopes overlap with the major neutralizing sites of the sl-cov s protein. these results demonstrated that sl-cov and sars-cov shared only a limited number of immunogenic epitopes in their s proteins and the major neutralization epitopes are substantially different. this work provides useful information for future development of differential serologic diagnosis and vaccines for coronaviruses with different s protein sequences. (c) 2009 elsevier inc. all rights reserved. |
| WOS关键词 | RECEPTOR-BINDING DOMAIN ; NEUTRALIZING ANTIBODIES ; ORIGIN ; COV |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000268978200020 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375573 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Shi, Zhengli |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Australian Biosecur Cooperat Res Ctr Emerging Inf, Australian Anim Hlth Lab, CSIRO Livestock Ind, Geelong, Vic, Australia |
| 推荐引用方式 GB/T 7714 | Zhou, Peng,Han, Zhenggang,Wang, Lin-Fa,et al. Immunogenicity difference between the sars coronavirus and the bat sars-like coronavirus spike (s) proteins[J]. Biochemical and biophysical research communications,2009,387(2):326-329. |
| APA | Zhou, Peng,Han, Zhenggang,Wang, Lin-Fa,&Shi, Zhengli.(2009).Immunogenicity difference between the sars coronavirus and the bat sars-like coronavirus spike (s) proteins.Biochemical and biophysical research communications,387(2),326-329. |
| MLA | Zhou, Peng,et al."Immunogenicity difference between the sars coronavirus and the bat sars-like coronavirus spike (s) proteins".Biochemical and biophysical research communications 387.2(2009):326-329. |
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来源：武汉病毒研究所
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