Genome-wide identification of binding sites for kaposi's sarcoma-associated herpesvirus lytic switch protein, rta
文献类型：期刊论文
| 作者 | Chen, Jiguo1,2; Ye, Fengchun1,2; Xie, Jianping1,2; Kuhne, Kurt1,3; Gao, Shou-Jiang1,2,3,4,5 |
| 刊名 | Virology
 |
| 出版日期 | 2009-04-10 |
| 卷号 | 386期号:2页码:290-302 |
| 关键词 | Kshv/hhv8 Replication and transcription activator (rta) Transcriptional regulation Whole-genome tiling microarray Chip-on-chip |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2009.01.031 |
| 通讯作者 | Gao, shou-jiang(gaos@uthscsa.edu) |
| 英文摘要 | Kaposi's sarcoma-associated herpesvirus (kshv) replication and transcription activator (rta) encoded by orf50 is a lytic switch protein for viral reactivation from latency. the expression of rta activates the expression of downstream viral genes, and is necessary for triggering the full viral lytic program. using chromatin immunoprecipitation assay coupled with a kshv whole-genome tiling, microarray (chip-on-chip) approach, we identified a set of 19 rta binding sites in the kshv genome in a kshv-infected cell line bcbl-1. these binding sites are located in the regions of promoters, introns, or exons of kshv genes including orf8, orfk4.1, orfk5, pan, orf16, orf29, orf45, orf50, orfk8, orfk10.1, orf59, orfk12, orf71/72, orfk14/orf74, and orfk15, the two origins of lytic replication orilyt-l and orilyt-r, and the microrna cluster. we confirmed these rta binding sites by chip and quantitative real-time pcr. we further mapped the rta binding site in the first intron of the orfk15 gene, and determined that it is rta-responsive. the orfk15 rta binding sequence ttccaggaa ttcctggaa consists of a palindromic structure of two tandem repeats, of which each itself is also an imperfect inverted repeat. reporter assay and electrophoretic mobility shift assay confirmed the binding of the rta protein to this sequence in vitro. sequence alignment with other rta binding sites identified the rta consensus binding motif as ttccaggat(n)(0-16)ttcctggga. interestingly, most of the identified rta binding sites contain only half or part of this rta binding motif. these results suggest the complexity of rta binding in vivo, and the involvement of other cellular or viral transcription factors during rta transactivation of target genes. (c) 2009 elsevier inc. all rights reserved. |
| WOS关键词 | DEPENDENT DNA-REPLICATION ; POLYADENYLATED NUCLEAR-RNA ; PRIMARY EFFUSION LYMPHOMA ; ORF50 RESPONSE ELEMENT ; DELAYED-EARLY PROMOTER ; READING FRAME 50 ; RBP-J-KAPPA ; TRANSCRIPTIONAL REGULATION ; GENE-EXPRESSION ; VIRAL REACTIVATION |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000267310200007 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375586 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Gao, Shou-Jiang |
| 作者单位 | 1.Univ Texas Hlth Sci Ctr San Antonio, Greehey Childrens Canc Res Inst, Tumor Virol Program, San Antonio, TX 78229 USA 2.Univ Texas Hlth Sci Ctr San Antonio, Dept Pediat, San Antonio, TX 78229 USA 3.Univ Texas Hlth Sci Ctr San Antonio, Dept Microbiol & Immunol, San Antonio, TX 78229 USA 4.Univ Texas Hlth Sci Ctr San Antonio, Canc Therapy & Res Ctr, San Antonio, TX 78229 USA 5.Chinese Acad Sci, Wuhan Inst Virol, Tumor Virol Grp, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Jiguo,Ye, Fengchun,Xie, Jianping,et al. Genome-wide identification of binding sites for kaposi's sarcoma-associated herpesvirus lytic switch protein, rta[J]. Virology,2009,386(2):290-302. |
| APA | Chen, Jiguo,Ye, Fengchun,Xie, Jianping,Kuhne, Kurt,&Gao, Shou-Jiang.(2009).Genome-wide identification of binding sites for kaposi's sarcoma-associated herpesvirus lytic switch protein, rta.Virology,386(2),290-302. |
| MLA | Chen, Jiguo,et al."Genome-wide identification of binding sites for kaposi's sarcoma-associated herpesvirus lytic switch protein, rta".Virology 386.2(2009):290-302. |
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来源：武汉病毒研究所
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