Characterization of the nuclear and nucleolar localization signals of bovine herpesvirus-1 infected cell protein 27
文献类型：期刊论文
| 作者 | Guo, Hong1; Ding, Qiong1; Lin, Fusen1; Pan, Weiwei1; Lin, Jianyin2; Zheng, Alan C.1 |
| 刊名 | Virus research
 |
| 出版日期 | 2009-11-01 |
| 卷号 | 145期号:2页码:312-320 |
| 关键词 | Nuclear localization signal Nucleolar localization signal Bovine herpesvirus-1 Bicp27 Transactivation |
| ISSN号 | 0168-1702 |
| DOI | 10.1016/j.virusres.2009.07.024 |
| 通讯作者 | Zheng, alan c.(zheng.alan@hotmail.com) |
| 英文摘要 | Bovine herpesvirus-1 infected cell protein 27 (bicp27) was detected predominantly in the nucleolus. the open reading frame of bicp27 was fused with the enhanced yellow fluorescent protein (eyfp) gene to investigate its subcellular localization in live cells and bicp27 was able to direct monomeric, dimeric or trimeric eyfp exclusively to the nucleolus. by constructing a series of deletion mutants, the putative nuclear localization signal (nls) and nucleolar localization signal (nols) were mapped to (81)rrar(84) and (86)rprrprrrprrr(97) respectively. specific deletion of the putative nls, nols or both abrogated nuclear localization, nucleolar localization or both respectively. furthermore, nls was able to direct trimeric eyfp predominantly to the nucleus but excluded from the nucleolus, whereas nols targeted trimeric eyfp primarily to the nucleus, and enriched in the nucleolus with faint staining in the cytoplasm. nls + nols directed trimeric eyfp predominantly to the nucleolus with faint staining in the nucleus. moreover, deletion of nls + nols abolished the transactivating activity of bicp27 on gc promoter, whereas deletion of either nls or nols did not. the study demonstrated that bicp27 is a nucleolar protein, adding bicp27 to the growing list of transactivators which localize to the nucleolus. (c) 2009 elsevier b.v. all rights reserved. |
| WOS关键词 | VIRUS REGULATORY PROTEIN ; TRANSACTIVATOR PROTEIN ; EXPORT SIGNALS ; GENE-PRODUCT ; TAT PROTEIN ; REV PROTEIN ; TYPE-1 REV ; RNA ; IDENTIFICATION ; SEQUENCE |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000271054900017 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375588 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zheng, Alan C. |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Mol Virol & Viral Immunol Res Grp, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 2.Fujian Med Univ, Dept Mol Med, Fuzhou 350001, Fujian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Hong,Ding, Qiong,Lin, Fusen,et al. Characterization of the nuclear and nucleolar localization signals of bovine herpesvirus-1 infected cell protein 27[J]. Virus research,2009,145(2):312-320. |
| APA | Guo, Hong,Ding, Qiong,Lin, Fusen,Pan, Weiwei,Lin, Jianyin,&Zheng, Alan C..(2009).Characterization of the nuclear and nucleolar localization signals of bovine herpesvirus-1 infected cell protein 27.Virus research,145(2),312-320. |
| MLA | Guo, Hong,et al."Characterization of the nuclear and nucleolar localization signals of bovine herpesvirus-1 infected cell protein 27".Virus research 145.2(2009):312-320. |
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来源：武汉病毒研究所
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