Cloning and characterization of nad-dependent protein deacetylase (rv1151c) from mycobacterium tuberculosis
文献类型：期刊论文
| 作者 | Gu, Jing1,2; Deng, Jiao-Yu1; Li, Ru1,2; Wei, Hongping1; Zhang, Zhiping1; Zhou, Yafeng1; Zhang, Ying3; Zhang, Xian-En1 |
| 刊名 | Biochemistry-moscow
 |
| 出版日期 | 2009-07-01 |
| 卷号 | 74期号:7页码:743-748 |
| 关键词 | Adp-ribosyltransferase Deacetylase Mycobacterium tuberculosis Nad glycohydrolase Sir2 |
| ISSN号 | 0006-2979 |
| DOI | 10.1134/s0006297909070062 |
| 通讯作者 | Zhang, xian-en(x.zhang@wh.iov.cn) |
| 英文摘要 | Sir2 family proteins are highly conserved and catalyze a well-characterized nad-dependent protein deacetylation reaction that regulates multiple cellular processes including aging, gene silencing, cellular differentiation, and metabolic pathways. little is known about sir2 family proteins in bacteria. the sir2 homolog rv1151c of mycobacterium tuberculosis was cloned and over-expressed in escherichia coli, and the protein then purified by ni(2+)-affinity chromatography to homogeneity. the purified recombinant protein showed a typical nad-dependent protein deacetylase activity that could be inhibited by nicotinamide and other known sir2 inhibitors. the optimal temperature and ph for activity of rv1151c are 25a degrees c and ph 9 +/- 1, respectively. rv1151c is capable of deacetylating the acetyl-coa synthetase from m. tuberculosis. however, unlike sir2 family proteins identified from other bacteria, rv1151c shows a substrate-independent nad glycohydrolase activity in accordance with its auto-adp ribosylation activity. |
| WOS关键词 | ADP-RIBOSYLTRANSFERASE ACTIVITY ; PLASMODIUM-FALCIPARUM SIR2 ; DNA-REPAIR ; YEAST SIR2 ; SACCHAROMYCES-CEREVISIAE ; HISTONE DEACETYLASE ; CALORIE RESTRICTION ; SIR2-LIKE PROTEINS ; TRYPANOSOMA-BRUCEI ; LIFE-SPAN |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000268256500006 |
| 出版者 | MAIK NAUKA/INTERPERIODICA/SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375589 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Xian-En |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD 21205 USA |
| 推荐引用方式 GB/T 7714 | Gu, Jing,Deng, Jiao-Yu,Li, Ru,et al. Cloning and characterization of nad-dependent protein deacetylase (rv1151c) from mycobacterium tuberculosis[J]. Biochemistry-moscow,2009,74(7):743-748. |
| APA | Gu, Jing.,Deng, Jiao-Yu.,Li, Ru.,Wei, Hongping.,Zhang, Zhiping.,...&Zhang, Xian-En.(2009).Cloning and characterization of nad-dependent protein deacetylase (rv1151c) from mycobacterium tuberculosis.Biochemistry-moscow,74(7),743-748. |
| MLA | Gu, Jing,et al."Cloning and characterization of nad-dependent protein deacetylase (rv1151c) from mycobacterium tuberculosis".Biochemistry-moscow 74.7(2009):743-748. |
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来源：武汉病毒研究所
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