Transcriptional up-regulation of foxm1 in response to hypoxia is mediated by hif-1
文献类型：期刊论文
| 作者 | Xia, Li-Min1; Huang, Wen-Jie2; Wang, Bo1; Liu, Mei1; Zhang, Qiong1; Yan, Wei1; Zhu, Qian1; Luo, Min1; Zhou, Zhen-Zhen1; Tian, De-An1 |
| 刊名 | Journal of cellular biochemistry
 |
| 出版日期 | 2009-02-01 |
| 卷号 | 106期号:2页码:247-256 |
| 关键词 | Foxm1 Hypoxia Hif-1 Promoter |
| ISSN号 | 0730-2312 |
| DOI | 10.1002/jcb.21996 |
| 通讯作者 | Tian, de-an(datian@tjh.tjmu.edu.cn) |
| 英文摘要 | The proliferation-specific forkhead box m i (fox m i) transcription factor is overexpressed in cancer cells a not acts as an important regulation of cancer cell growth and survival. here, we show the molecular mechanisms by which hypoxia regulate foxm1 expression in cancer cells. when cells were subjected to hypoxia (1% o(2)), the mrna and protein levels of foxm1 had a significant increase in cancer cells (hepg2, mcf-7, and hela). such increase was due to the direct binding of hypoxia-inducible factor i (hif-1) to the hif-1 binding sites in the foxm1 promoter. by deletion and mutation assays, we demonstrated that the hif1-1 and hif1-3/4 binding sites on the foxm1 promoter were essential for transcriptional activation of foxm1 by hypoxia. we also demonstrated that hif-1 alpha directly bound to the promoter of foxm1 and the binding was specific, as revealed by hif-1 binding/competition assay and chromatin immunoprecipitation assay. consequently, the up-regulation of foxm1 accelerated the growth of hypoxic cancer cells by decreasing nuclear levels of p21 and increasing expression of cyclin b1 and cyclin d1. these findings provide a new insight into how tumor cells overcome hypoxic stress and survive, and also disclose a new regulatory mechanism of foxm1 expression in tumor cells. j. cell. biochem. 106: 247-256, 2009. (c) 2008 wiley-liss, inc. |
| WOS关键词 | INDUCIBLE FACTOR-1 ; HEPATOCELLULAR CARCINOMAS ; PROSTATE CARCINOMAS ; LIVER-REGENERATION ; DNA-REPLICATION ; GENE-EXPRESSION ; LUNG-CANCER ; FACTOR-I ; FORKHEAD ; PROTEIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000262921600007 |
| 出版者 | WILEY-LISS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375590 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Tian, De-An |
| 作者单位 | 1.Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Div Gastroenterol, Wuhan 430030, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Li-Min,Huang, Wen-Jie,Wang, Bo,et al. Transcriptional up-regulation of foxm1 in response to hypoxia is mediated by hif-1[J]. Journal of cellular biochemistry,2009,106(2):247-256. |
| APA | Xia, Li-Min.,Huang, Wen-Jie.,Wang, Bo.,Liu, Mei.,Zhang, Qiong.,...&Tian, De-An.(2009).Transcriptional up-regulation of foxm1 in response to hypoxia is mediated by hif-1.Journal of cellular biochemistry,106(2),247-256. |
| MLA | Xia, Li-Min,et al."Transcriptional up-regulation of foxm1 in response to hypoxia is mediated by hif-1".Journal of cellular biochemistry 106.2(2009):247-256. |
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来源：武汉病毒研究所
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