Genetic and biochemical analyses of chlorobenzene degradation gene clusters in pandoraea sp strain mcb032
文献类型：期刊论文
| 作者 | Jiang, Xi-Wen1; Liu, Hong1; Xu, Ying1; Wang, Shu-Jun1; Leak, David J.2; Zhou, Ning-Yi1 |
| 刊名 | Archives of microbiology
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 191期号:6页码:485-492 |
| 关键词 | Chlorobenzene Chlorocatechol Degradation Pandoraea |
| ISSN号 | 0302-8933 |
| DOI | 10.1007/s00203-009-0476-9 |
| 通讯作者 | Zhou, ning-yi(n.zhou@pentium.whiov.ac.cn) |
| 英文摘要 | Pandoraea sp. strain mcb032 was isolated as an emerging chlorobenzene degrader from a functionally stable bioreactor where species succession had occurred. in this study, two gene clusters encoding chlorobenzene metabolic functions have been cloned. within the cbs gene cluster, cbsa and cbsb are similar to the chlorobenzene dioxygenase and the cis-chlorobenzene dihydrodiol dehydrogenase in ralstonia sp. js705 and shown to transform chlorobenzene to 3-chlorocatechol. the clc gene cluster shows strong similarity to the clc genes of ralstonia sp. js705 and encodes chlorocatechol 1,2-dioxygenase (clca) and other enzymes, which catalyze the conversion of chlorocatechol to 3-oxoadipate. the michaelis constants (k (m)) values of clca for catechol, 3-methylcatechol and 3-chlorocatechol were determined as 10.0, 8.9 and 3.4 mu m, respectively. cbsx, a putative transport protein present in the cbs cluster of strain mcb032 but not in those of other chlorobenzene degraders, shows 76 and 53% identities to two previously identified transport proteins involved in toluene degradation, tbux from ralstonia pickettii pko1 and todx from pseudomonas putida f1. the presence of the transport protein in strain mcb032 likely provides a mechanistic explanation for its higher chlorobenzene affinity and may well be the basis for the competitive advantage of this strain in the bioreactor. |
| WOS关键词 | PSEUDOMONAS SP ; DIHYDRODIOL DEHYDROGENASE ; CONTAMINATED GROUNDWATER ; TOLUENE DEGRADATION ; PICKETTII PKO1 ; GENOMIC ISLAND ; SP NOV. ; PATHWAY ; BIODEGRADATION ; B13 |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000266260500001 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375595 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhou, Ning-Yi |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Univ London Imperial Coll Sci Technol & Med, Div Biol, Fac Nat Sci, London SW7 2AZ, England |
| 推荐引用方式 GB/T 7714 | Jiang, Xi-Wen,Liu, Hong,Xu, Ying,et al. Genetic and biochemical analyses of chlorobenzene degradation gene clusters in pandoraea sp strain mcb032[J]. Archives of microbiology,2009,191(6):485-492. |
| APA | Jiang, Xi-Wen,Liu, Hong,Xu, Ying,Wang, Shu-Jun,Leak, David J.,&Zhou, Ning-Yi.(2009).Genetic and biochemical analyses of chlorobenzene degradation gene clusters in pandoraea sp strain mcb032.Archives of microbiology,191(6),485-492. |
| MLA | Jiang, Xi-Wen,et al."Genetic and biochemical analyses of chlorobenzene degradation gene clusters in pandoraea sp strain mcb032".Archives of microbiology 191.6(2009):485-492. |
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来源：武汉病毒研究所
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