Visualization of the dynamic multimerization of human cytomegalovirus pp65 in punctuate nuclear foci
文献类型：期刊论文
| 作者 | Cui, Zongqiang1; Zhang, Ke1,2; Zhang, Zhiping1; Liu, Yalan1,2; Zhou, Yafeng1; Wei, Hongping1; Zhang, Xian-En1 |
| 刊名 | Virology
 |
| 出版日期 | 2009-09-30 |
| 卷号 | 392期号:2页码:169-177 |
| 关键词 | Hcmv pp65 Dynamic accumulation Nuclear foci Self-interact Frap Fret |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2009.06.021 |
| 通讯作者 | Zhang, xian-en(x.zhang@wh.iov.cn) |
| 英文摘要 | The phosphorylated protein pp65 of human cytomegalovirus (hcmv) is the predominant virion protein and the major tegument constituent. it plays important roles in hcmv infection and virion assembly. live cell imaging and fluorescence recovery after photobleaching (frap) analysis showed that hcmv pp65 accumulated dynamically ill punctuate nuclear foci when transiently expressed in mammalian cells. fluorescence resonance energy transfer (fret) imaging disclosed that pp65 can self-interact in its localization foci. yeast two-hybrid assay verified that pp65 is a self-associating protein, and the n-terminal amino acids 14-22 were determined to be essential for pp65 self-association. however, these amino acids were not related to pp65 localization in the specific nuclear foci. the interaction of pp65 and ppul97 was also studied by fret microscopy, and the result suggested that there is another signal sequence in pp65, being the ppul97 phosphorylation site, that is responsible for localization of pp65 in nuclear foci. these results help to understand the function of pp65 in hcmv infection and virion morphogenesis. (c) 2009 elsevier inc. all rights reserved. |
| WOS关键词 | TEGUMENT PROTEIN PP65 ; RESONANCE ENERGY-TRANSFER ; STRUCTURAL PROTEINS ; LOCALIZATION ; KINASE ; MATRIX ; VIRION ; REPLICATION ; UL83 ; FIBROBLASTS |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000270247300003 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375598 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Xian-En |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Zongqiang,Zhang, Ke,Zhang, Zhiping,et al. Visualization of the dynamic multimerization of human cytomegalovirus pp65 in punctuate nuclear foci[J]. Virology,2009,392(2):169-177. |
| APA | Cui, Zongqiang.,Zhang, Ke.,Zhang, Zhiping.,Liu, Yalan.,Zhou, Yafeng.,...&Zhang, Xian-En.(2009).Visualization of the dynamic multimerization of human cytomegalovirus pp65 in punctuate nuclear foci.Virology,392(2),169-177. |
| MLA | Cui, Zongqiang,et al."Visualization of the dynamic multimerization of human cytomegalovirus pp65 in punctuate nuclear foci".Virology 392.2(2009):169-177. |
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来源：武汉病毒研究所
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