The tegument protein ul94 of human cytomegalovirus as a binding partner for tegument protein pp28 identified by intracellular imaging
文献类型：期刊论文
| 作者 | Liu, Yalan1,2; Cui, Zongqiang1; Zhang, Zhiping1; Wei, Hongping1; Zhou, Yafeng1; Wang, Mingli3; Zhang, Xian-En1 |
| 刊名 | Virology
 |
| 出版日期 | 2009-05-25 |
| 卷号 | 388期号:1页码:68-77 |
| 关键词 | Hcmv Tegument protein Pp28 Ul94 Fret Intracellular image Protein interaction |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2009.03.007 |
| 通讯作者 | Zhang, xian-en(x.zhang@wh.iov.cn) |
| 英文摘要 | The tegument protein pp28 of human cytomegalovirus (hcmv) is essential for the assembly of infectious hcmv virions, but how it functions during the process of hcmv tegumentation and envelopment remains unclear. by using live cell fluorescence resonance energy transfer (fret) microscopy and yeast two-hybrid assays, we found that another hcmv tegument protein, ul94, was a specific binding partner for pp28. the interaction between pp28 and ul94 was imaged in a punctuate, juxtanuclear compartment, previously designated as the virus assembly compartment (ac). amino acids 22-43 of pp28 were identified as being responsible for its binding with ul94, while no linear binding site could be found within ul94. the interaction between pp28 and ul94 may serve as a link in the sequential processes of hcmv capsidation, tegumentation and envelopment. this study provides a foundation for further studies into how the hcmv tegument proteins act in the assembly of hcmv virions. (c) 2009 elsevier inc. all rights reserved. |
| WOS关键词 | HERPES-SIMPLEX-VIRUS ; CYTOPLASMIC COMPARTMENT ; INFECTIOUS VIRUS ; EXPRESSION ; SEQUENCE ; VIRION ; LOCALIZATION ; REPLICATION ; PARTICLES ; CAPSIDS |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000266288700009 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375599 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Xian-En |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Anhui Med Univ, Dept Microbiol, Hefei 230032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yalan,Cui, Zongqiang,Zhang, Zhiping,et al. The tegument protein ul94 of human cytomegalovirus as a binding partner for tegument protein pp28 identified by intracellular imaging[J]. Virology,2009,388(1):68-77. |
| APA | Liu, Yalan.,Cui, Zongqiang.,Zhang, Zhiping.,Wei, Hongping.,Zhou, Yafeng.,...&Zhang, Xian-En.(2009).The tegument protein ul94 of human cytomegalovirus as a binding partner for tegument protein pp28 identified by intracellular imaging.Virology,388(1),68-77. |
| MLA | Liu, Yalan,et al."The tegument protein ul94 of human cytomegalovirus as a binding partner for tegument protein pp28 identified by intracellular imaging".Virology 388.1(2009):68-77. |
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来源：武汉病毒研究所
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