Rapid detection of bacillus anthracis using monoclonal antibody functionalized qcm sensor
文献类型：期刊论文
| 作者 | Hao, Rongzhang2; Wang, Dianbing1,3; Zhang, Xian'en1; Zuo, Guomin2; Wei, Hongping1; Yang, Ruifu4; Zhang, Zhiping1; Cheng, Zhenxing2; Guo, Yongchao1; Cui, Zongqiang1 |
| 刊名 | Biosensors & bioelectronics
 |
| 出版日期 | 2009 |
| 卷号 | 24期号:5页码:1330-1335 |
| 关键词 | Piezoelectric immunosensor Bacillus anthracis Monoclonal antibody (mab) Quartz crystal microbalance (qcm) Mixed self-assembled monolayer (sam) Protein a |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2008.07.071 |
| 通讯作者 | Zhang, zhiping(zhangzp@wh.iov.cn) |
| 英文摘要 | Since the anthrax spore bioterrorism attacks in america in 2001, the early detection of bacillus anthracis spores and vegetative cells has gained significant interest. at present, many polyclonal antibody-based quartz crystal microbalance (qcm) sensors have been developed to detect b. anthracis simulates. to achieve a simultaneous rapid detection of b. anthracis spores and vegetative cells, this paper presents a biosensor that utilizes an anti-b. anthracis monoclonal antibody designated to 8g3 (mab 8c3, igg) functionalized qcm sensor. having compared four kinds of antibody immobilizations on au surface, an optimized mab 8g3 was immobilized onto the au electrode with protein a on a mixed self-assembled monolayer (sam) of 11-mercaptoundecanoic acid (11-mua) and 6-mercaptohexan-1-ol (6-mho) as adhesive layer. the detection of b. anthracis was investigated under three conditions: dip-and-dry, static addition and flow through procedure. the results indicated that the sensor yielded a distinct response to b. anthracis spores or vegetative cells but had no significant response to bacillus thuringiensis species. the functionalized sensor recognized b. anthracis spores and vegetative cells specifically from its homophylic ones, and the limit of detection (lod) reached 10(3) cfu or spores/ml of b. anthracis in less than 30 min. cyclic voltammogram (cv) and scanning electronic microscopy (sem) were performed to characterize the surface of the sensor in variable steps during the modification and after the detection. the mab functionalized qcm biosensor will be helpful in the fabrication of a similar biosensor that may be available in anti-bioterrorism in the future. (c) 2008 elsevier b.v. all rights reserved. |
| WOS关键词 | QUARTZ-CRYSTAL MICROBALANCE ; PIEZOELECTRIC IMMUNOSENSOR ; BIOSENSOR APPLICATIONS ; PEMC SENSORS ; IN-SITU ; IMMOBILIZATION ; IMMUNOASSAY ; STABILIZATION ; BIOTERRORISM ; STRATEGIES |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| WOS记录号 | WOS:000263199800045 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375600 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Zhiping |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Inst Chem Def, Dept 3, Beijing 102205, Peoples R China 3.Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agromicrobiol, Wuhan 430070, Peoples R China 4.Acad Mil Med Sci, Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecurity, Beijing 100071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, Rongzhang,Wang, Dianbing,Zhang, Xian'en,et al. Rapid detection of bacillus anthracis using monoclonal antibody functionalized qcm sensor[J]. Biosensors & bioelectronics,2009,24(5):1330-1335. |
| APA | Hao, Rongzhang.,Wang, Dianbing.,Zhang, Xian'en.,Zuo, Guomin.,Wei, Hongping.,...&Zhou, Yafeng.(2009).Rapid detection of bacillus anthracis using monoclonal antibody functionalized qcm sensor.Biosensors & bioelectronics,24(5),1330-1335. |
| MLA | Hao, Rongzhang,et al."Rapid detection of bacillus anthracis using monoclonal antibody functionalized qcm sensor".Biosensors & bioelectronics 24.5(2009):1330-1335. |
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来源：武汉病毒研究所
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