Characterization of glycerol dehydratase expressed by fusing its alpha- and beta-subunits
文献类型：期刊论文
| 作者 | Wang, Xu-De1,2; Zhang, Xian-En1; Guo, Yong-Chao1; Zhang, Zhi-Ping1; Cao, Zhu-An3; Zhou, Ya-Feng1 |
| 刊名 | Biotechnology letters
 |
| 出版日期 | 2009-05-01 |
| 卷号 | 31期号:5页码:711-717 |
| ISSN号 | 0141-5492 |
| 关键词 | Fusion expression Glycerol dehydratase Glycerol dehydratase reactivase Klebsiella pneumoniae 1,3-propanediol |
| DOI | 10.1007/s10529-009-9911-x |
| 通讯作者 | Zhou, ya-feng(zyf@wh.iov.cn) |
| 英文摘要 | The gdh and gdhr genes, encoding b(12)-dependent glycerol dehydratase (gdh) and glycerol dehydratase reactivase (gdhr), respectively, in klebsiella pneumoniae, were cloned and expressed in e. coli. part of the beta-subunit was lost during gdh purification when co-expressing alpha, beta and gamma subunit. this was overcome by fusing the beta-subunit to alpha- or gamma-subunit with/without the insertion of a linker peptide between the fusion moieties. the kinetic properties of the fusion enzymes were characterized and compared with wild type enzyme. the results demonstrated that the fusion protein gdhalb/c, constructed by linking the n-terminal of beta-subunit to the c-terminal of alpha subunit through a (gly(4)ser)(4) linker peptide, had the greatest catalytic activity. similar to the wild-type enzyme, gdhalb/c underwent mechanism-based inactivation by glycerol during catalysis and could be reactivated by gdhr. |
| WOS关键词 | DEPENDENT DIOL DEHYDRASE ; KLEBSIELLA-PNEUMONIAE ; AEROBACTER-AEROGENES ; REACTIVATING FACTOR ; ADENOSYLCOBALAMIN ; COENZYME ; MECHANISM ; 1,3-PROPANEDIOL ; CLONING ; ANALOGS |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000265084500014 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375604 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhou, Ya-Feng |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Tsinghua Univ, Dept Chem Engn, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xu-De,Zhang, Xian-En,Guo, Yong-Chao,et al. Characterization of glycerol dehydratase expressed by fusing its alpha- and beta-subunits[J]. Biotechnology letters,2009,31(5):711-717. |
| APA | Wang, Xu-De,Zhang, Xian-En,Guo, Yong-Chao,Zhang, Zhi-Ping,Cao, Zhu-An,&Zhou, Ya-Feng.(2009).Characterization of glycerol dehydratase expressed by fusing its alpha- and beta-subunits.Biotechnology letters,31(5),711-717. |
| MLA | Wang, Xu-De,et al."Characterization of glycerol dehydratase expressed by fusing its alpha- and beta-subunits".Biotechnology letters 31.5(2009):711-717. |
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来源：武汉病毒研究所
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