Generation and evaluation of an h9n1 influenza vaccine derived by reverse genetics that allows utilization of a diva strategy for control of h9n2 avian influenza
文献类型：期刊论文
| 作者 | Wu, Rui1,2; Chen, Quanjiao1; Zheng, Liyun1; Chen, Jianjun1; Sui, Zhiwei1; Guan, Yi3; Chen, Ze1,4,5 |
| 刊名 | Archives of virology
 |
| 出版日期 | 2009-08-01 |
| 卷号 | 154期号:8页码:1203-1210 |
| ISSN号 | 0304-8608 |
| DOI | 10.1007/s00705-009-0425-6 |
| 通讯作者 | Chen, ze(chenze2005@hotmail.com) |
| 英文摘要 | H9n2 avian influenza viruses have circulated widely in domestic poultry around the world, and their outbreaks have resulted in heavy morbidity and mortality. in addition, h9n2 avian influenza viruses were transmitted directly from birds to humans in hong kong and mainland china during 1998 and 2003, which prompted the public health authorities to seek protective strategies to control h9n2 influenza viruses. in this study, we attempted to develop a diva (differentiating infected and vaccinated animals) strategy for h9n2 avian influenza viruses. this strategy does not interfere with serological monitoring and allows effective control of h9n2 avian influenza. we generated a reassortant h9n1 influenza vaccine strain by reverse genetics and employed an enzyme-linked immunosorbent assay ( elisa) with a truncated n1 antigen expressed in e. coli to differentiate between vaccinated and naturally infected animals. immunization of balb/c mice with the inactivated reassortant h9n1 vaccine conferred protection against lethal challenge with h9n2 viruses. meanwhile, the elisa can be used to distinguish between vaccination and natural infection quickly and easily. therefore, this study has opened up a new avenue for the control of h9n2 avian influenza. |
| WOS关键词 | A VIRUS ; HONG-KONG ; ANIMALS STRATEGY ; HUMAN INFECTION ; DUCKS ; NEURAMINIDASE ; PROTECTION ; CHALLENGE ; CANDIDATE ; CHICKENS |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000269687400003 |
| 出版者 | SPRINGER WIEN |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375605 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Ze |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Univ Hong Kong, Dept Microbiol, Pokfulam, Hong Kong, Peoples R China 4.Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China 5.Shanghai Inst Biol Prod, Shanghai 200052, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Rui,Chen, Quanjiao,Zheng, Liyun,et al. Generation and evaluation of an h9n1 influenza vaccine derived by reverse genetics that allows utilization of a diva strategy for control of h9n2 avian influenza[J]. Archives of virology,2009,154(8):1203-1210. |
| APA | Wu, Rui.,Chen, Quanjiao.,Zheng, Liyun.,Chen, Jianjun.,Sui, Zhiwei.,...&Chen, Ze.(2009).Generation and evaluation of an h9n1 influenza vaccine derived by reverse genetics that allows utilization of a diva strategy for control of h9n2 avian influenza.Archives of virology,154(8),1203-1210. |
| MLA | Wu, Rui,et al."Generation and evaluation of an h9n1 influenza vaccine derived by reverse genetics that allows utilization of a diva strategy for control of h9n2 avian influenza".Archives of virology 154.8(2009):1203-1210. |
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来源：武汉病毒研究所
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