Interaction of sortase a and lipase 2 in the inhibition of staphylococcus aureus biofilm formation
文献类型：期刊论文
| 作者 | Xiong, Ning1,2; Hu, Chunyan1,2; Zhang, Yong1; Chen, Shiyun1 |
| 刊名 | Archives of microbiology
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 191期号:12页码:879-884 |
| 关键词 | Staphylococcus aureus Sortase a Biofilm Lipase |
| ISSN号 | 0302-8933 |
| DOI | 10.1007/s00203-009-0520-9 |
| 通讯作者 | Chen, shiyun(sychen@wh.iov.cn) |
| 英文摘要 | Recombinant sortase a (srta) was used to immune rabbit, and the inhibitory activity of anti-srta serum on staphylococcus aureus biofilm formation was tested. biofilm formation was inhibited by anti-srta rabbit serum in s. aureus atcc25923 and two clinical isolated strains. the antiserum was separated into two fractions, and the main component with the inhibitory activity was demonstrated to be the igg fraction. two proteins interact with the igg fraction were identified by using an in vitro pull-down assay and were confirmed to be lipase 2 and gamma-hemolysin by mass spectrometry. cross-interaction between srta and lipase 2 was further confirmed by western blotting. addition of anti-lipase 2 serum in the culture medium also showed inhibitory effect against biofilm formation. together, our study suggests anti-srta serum inhibits s. aureus biofilm formation and lipase 2 is one of the targets of anti-srta serum in this inhibition process. this is the first study to demonstrate the roles of antisera against srta and lipase 2 in the inhibition of biofilm formation in s. aureus. |
| WOS关键词 | SURFACE-PROTEINS ; FIBRONECTIN ; IDENTIFICATION ; TRANSPEPTIDASE ; INFECTIONS ; VIRULENCE ; ADHESION ; MUTANTS ; VACCINE ; TARGET |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000271761200002 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375625 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Shiyun |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei Province, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiong, Ning,Hu, Chunyan,Zhang, Yong,et al. Interaction of sortase a and lipase 2 in the inhibition of staphylococcus aureus biofilm formation[J]. Archives of microbiology,2009,191(12):879-884. |
| APA | Xiong, Ning,Hu, Chunyan,Zhang, Yong,&Chen, Shiyun.(2009).Interaction of sortase a and lipase 2 in the inhibition of staphylococcus aureus biofilm formation.Archives of microbiology,191(12),879-884. |
| MLA | Xiong, Ning,et al."Interaction of sortase a and lipase 2 in the inhibition of staphylococcus aureus biofilm formation".Archives of microbiology 191.12(2009):879-884. |
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来源：武汉病毒研究所
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