Differential long non-coding rna and mrna expression in differentiated human glioblastoma stem cells
文献类型：期刊论文
| 作者 | Li, Hao1,2; Li, Haowen3; Hao, Yajing4; Jiao, Yuming1,2; Li, Zhicen1,2; Yue, Haiyan4; Xu, Zhe3; Wang, Shuo1,2; Cao, Yong1,2; Zhao, Jizong1,2 |
| 刊名 | Molecular medicine reports
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 14期号:3页码:2067-2076 |
| ISSN号 | 1791-2997 |
| 关键词 | Glioblastoma stem cells Glioblastoma multiforme Long non-coding rna Differentiation |
| DOI | 10.3892/mmr.2016.5505 |
| 通讯作者 | Cao, yong(caoyong6@hotmail.com) ; Zhao, jizong(zhaojz205@163.com) |
| 英文摘要 | Differentiation of glioblastoma stem cells (gscs) may lead to inhibition of their self-renewing ability and tumorigenic potential, as well as increasing their sensitivity to treatment. the critical role of long non-coding rnas (lncrnas) in numerous biological processes has been revealed. however, the involvement of lncrnas in gsc differentiation remains to be elucidated. in the present study, gscs were isolated from patient samples and differentiation was induced. using a high-throughput microarray, the present study identified a profile of 1,545 lncrnas and 2,729 mrnas that differed between gscs and their non-differentiated counterparts. to ascertain the association between the altered lncrnas and mrnas, a co-expression network was constructed in which 1,087 lncrnas and 1,928 mrnas altered upon gsc differentiation formed a total of 19,642 lncrna-mrna pairs. based on the co-expression network, the lncrna functions were additionally predicted by a cis- or trans- targeting program. furthermore, three pairs of lncrnas and their nearby target mrnas were selected [ensg00000261924.1-regulatory associated protein of mtor complex 1, ensg00000235427.1-caveolin 1 and tax1 binding protein 3 (tax1bp3)-purinergic receptor p2x 5 (p2rx5)-tax1bp3] and their expression levels were validated by reverse transcription-quantitative polymerase chain reaction. the altered lncrnas were also regulated by various pluripotency transcription factors (pou domain, class 3, transcription factor, sex determining region y-box 2, spalt-like transcription factor 2 and oligodendrocyte lineage transcription factor 2). in conclusion, the results of the present study revealed that lncrnas may function in gsc differentiation by regulating their target mrnas, and a set of lncrnas were identified as candidates for further study concerning the future treatment of gscs. |
| WOS关键词 | NEURONAL DIFFERENTIATION ; GLIOMA ; SIGNATURE ; TARGET ; PLURIPOTENCY ; ACTIVATION ; PHENOTYPES ; REVEALS |
| WOS研究方向 | Oncology ; Research & Experimental Medicine |
| WOS类目 | Oncology ; Medicine, Research & Experimental |
| 语种 | 英语 |
| 出版者 | SPANDIDOS PUBL LTD |
| WOS记录号 | WOS:000382445400026 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375633 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Cao, Yong; Zhao, Jizong |
| 作者单位 | 1.Capital Med Univ, Beijing Tiantan Hosp, Dept Neurosurg, 6 Tiantanxili, Beijing 100050, Peoples R China 2.China Natl Clin Res Ctr Neurol Dis, Beijing 100050, Peoples R China 3.Capital Med Univ, Beijing Tiantan Hosp, Lab Clin Med Res, Beijing 100050, Peoples R China 4.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Biophys, Lab Noncoding RNA, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Hao,Li, Haowen,Hao, Yajing,et al. Differential long non-coding rna and mrna expression in differentiated human glioblastoma stem cells[J]. Molecular medicine reports,2016,14(3):2067-2076. |
| APA | Li, Hao.,Li, Haowen.,Hao, Yajing.,Jiao, Yuming.,Li, Zhicen.,...&Zhao, Jizong.(2016).Differential long non-coding rna and mrna expression in differentiated human glioblastoma stem cells.Molecular medicine reports,14(3),2067-2076. |
| MLA | Li, Hao,et al."Differential long non-coding rna and mrna expression in differentiated human glioblastoma stem cells".Molecular medicine reports 14.3(2016):2067-2076. |
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来源：中国科学院大学
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