Partial functional rescue of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus infectivity by replacement of f protein with gp64 from autographa californica multicapsid nucleopolyhedrovirus
文献类型：期刊论文
| 作者 | Wang, Manli1,2; Yin, Feifei1,2; Shen, Shu1,2; Tan, Ying1,2; Deng, Fei1,2; Vlak, Just M.3; Hu, Zhihong1,2; Wang, Hualin1,2 |
| 刊名 | Journal of virology
 |
| 出版日期 | 2010-11-01 |
| 卷号 | 84期号:21页码:11505-11514 |
| ISSN号 | 0022-538X |
| DOI | 10.1128/jvi.00862-10 |
| 通讯作者 | Wang, hualin(h.wang@wh.iov.cn) |
| 英文摘要 | Two distinct envelope fusion proteins (efps) (gp64 and f) have been identified in members of the baculoviridae family of viruses. f proteins are found in group ii nucleopolyhedroviruses (npvs) of alphabaculoviruses and in beta- and deltabaculoviruses, while gp64 occurs only in group i npvs of alphabaculoviruses. it was proposed that an ancestral baculovirus acquired the gp64 gene that conferred a selective advantage and allowed it to evolve into group i npvs. the f protein is a functional analogue of gp64, as evidenced from the rescue of gp64-null autographa californica multicapsid nucleopolyhedrovirus (mnpv) (acmnpv) by f proteins from group ii npvs or from betabaculoviruses. however, gp64 failed to rescue an f-null spodoptera exigua mnpv (semnpv) (group ii npv). here, we report the successful generation of an infectious gp64-rescued group ii npv of helicoverpa armigera (vhabac delta f-gp64). viral growth curve assays and quantitative real-time pcr (q-pcr), however, showed substantially decreased infectivity of vhabac delta f-gp64 compared to the haf rescue control virus vhabac delta f-haf. electron microscopy further showed that most vhabac delta f-gp64 budded viruses (bv) in the cell culture supernatant lacked envelope components and contained morphologically aberrant nucleocapsids, suggesting the improper bv envelopment or budding of vhabac delta f-gp64. bioassays using pseudotyped viruses with a reintroduced polyhedrin gene showed that gp64-pseudotyped helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (hearnpv) significantly delayed the mortality of infected h. armigera larvae. |
| WOS关键词 | ENVELOPE FUSION PROTEIN ; NUCLEAR POLYHEDROSIS-VIRUS ; BACULOVIRUS GP64 ; LENTIVIRAL VECTORS ; MEMBRANE-FUSION ; GLYCOPROTEINS ; CELLS ; HOMOLOG ; ENTRY ; AC23 |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000282643400054 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375674 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Hualin |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China 3.Wageningen Univ, Virol Lab, NL-6708 PB Wageningen, Netherlands |
| 推荐引用方式 GB/T 7714 | Wang, Manli,Yin, Feifei,Shen, Shu,et al. Partial functional rescue of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus infectivity by replacement of f protein with gp64 from autographa californica multicapsid nucleopolyhedrovirus[J]. Journal of virology,2010,84(21):11505-11514. |
| APA | Wang, Manli.,Yin, Feifei.,Shen, Shu.,Tan, Ying.,Deng, Fei.,...&Wang, Hualin.(2010).Partial functional rescue of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus infectivity by replacement of f protein with gp64 from autographa californica multicapsid nucleopolyhedrovirus.Journal of virology,84(21),11505-11514. |
| MLA | Wang, Manli,et al."Partial functional rescue of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus infectivity by replacement of f protein with gp64 from autographa californica multicapsid nucleopolyhedrovirus".Journal of virology 84.21(2010):11505-11514. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。